显微外科动静脉环与生物模板：一种用于组织工程的新型体内腔室。
Microsurgical arterovenous loops and biological templates: a novel in vivo chamber for tissue engineering.
作者信息
Manasseri Benedetto, Cuccia Giuseppe, Moimas Silvia, D'Alcontres Francesco Stagno, Polito Francesca, Bitto Alessandra, Altavilla Domenica, Squadrito Francesco, Geuna Stefano, Pattarini Lucia, Zentilin Lorena, Collesi Chiara, Puligadda Uday, Giacca Mauro, Colonna Michele Rosario
机构信息
Department of Surgical Specialties, Plastic Surgery Division, University of Messina Medical School, Messina, Italy.
出版信息
Microsurgery. 2007;27(7):623-9. doi: 10.1002/micr.20415.
BACKGROUND
Microsurgical tissue engineering is an emerging topic in regenerative medicine. Here we describe a new microsurgical model of bioengineering in rats based on the use of an arterovenous loop (AV) implanted into a commercially available crosslinked collagen/glycosaminoglycan template.
METHODS
The microvascular loop was created between the femoral artery and vein and covered by the template folded onto itself. The chamber was isolated from the outside tissue by an outer silicon layer to impede tissue ingrowth.
RESULTS
At 1-month postimplantation, the tissue chamber was found heavily vascularized, as assessed by laser Doppler perfusion analysis. Histological examination showed that the AV loop was integrated into the collagen matrix of the template and that the whole template was filled with a newly formed soft connective tissue. Most interestingly, the whole scaffold was found heavily vascularized, including the formation of a large number of alpha-SMA-positive arterioles.
CONCLUSIONS
The developed microsurgical chamber provides a highly vascular, isolated tool for in vivo tissue engineering.
背景
显微外科组织工程是再生医学中一个新兴的课题。在此，我们描述一种基于将动静脉环（AV）植入市售交联胶原/糖胺聚糖模板的大鼠生物工程新显微外科模型。
方法
在股动脉和静脉之间构建微血管环，并用自身折叠的模板覆盖。通过外部硅层将腔室与外部组织隔离，以阻止组织向内生长。
结果
植入后1个月，通过激光多普勒灌注分析评估发现组织腔室血管化程度很高。组织学检查显示动静脉环整合到模板的胶原基质中，并且整个模板充满了新形成的软结缔组织。最有趣的是，发现整个支架血管化程度很高，包括形成大量α-SMA阳性小动脉。
结论
所开发的显微外科腔室为体内组织工程提供了一种高度血管化的隔离工具。
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