de Ruiter Godard C, Spinner Robert J, Alaid Awad O, Koch Anthony J, Wang Huan, Malessy Martijn J A, Currier Bradford L, Yaszemski Michael J, Kaufman Kenton R, Windebank Anthony J
Department of Neurologic Surgery, Mayo Clinic, Rochester, MN 55905, USA.
J Peripher Nerv Syst. 2007 Sep;12(3):216-22. doi: 10.1111/j.1529-8027.2007.00142.x.
Ankle motion analysis may provide a better method to assess function in the rat sciatic nerve model than the standard method, the sciatic functional index (SFI), but it is not widely used in experiments on nerve regeneration possibly because of complicated analysis. In this study, we investigated the practical use of a two-dimensional (2D) digital video motion analysis system. Reproducibility was investigated in normal rats. Recovery of ankle motion was analyzed after sciatic, tibial, and peroneal nerve crush injury. Results were compared with scores for the SFI. Results were not significantly different from animal-to-animal and day-to-day. Interobserver variability also was small. In the analysis of recovery after separate nerve crush injuries, subtle differences in ankle plantar flexion and dorsiflexion could be detected. The method was also more sensitive than the SFI: whereas scores for the SFI had returned to normal 4 weeks after sciatic nerve crush injury, the ankle angle at mid-stance was still significantly different from that in sham-operated animals 6 weeks after the injury. 2D digital video ankle motion analysis is a practical and sensitive method to assess function in the rat sciatic nerve model.
与标准方法坐骨神经功能指数（SFI）相比，踝关节运动分析可能为评估大鼠坐骨神经模型的功能提供一种更好的方法，但它可能由于分析复杂而未在神经再生实验中广泛应用。在本研究中，我们调查了二维（2D）数字视频运动分析系统的实际应用情况。我们在正常大鼠中研究了该系统的可重复性。在坐骨神经、胫神经和腓总神经挤压损伤后，分析了踝关节运动的恢复情况。将结果与SFI评分进行比较。不同动物之间以及不同日期之间的结果无显著差异。观察者间的变异性也很小。在单独神经挤压损伤后的恢复分析中，可以检测到踝关节跖屈和背屈的细微差异。该方法也比SFI更敏感：坐骨神经挤压损伤后4周，SFI评分已恢复正常，而损伤后6周，站立中期的踝关节角度仍与假手术动物的角度有显著差异。二维数字视频踝关节运动分析是评估大鼠坐骨神经模型功能的一种实用且敏感的方法。
