Bozkurt Ahmet, Tholl Sacha, Wehner Sarah, Tank Julian, Cortese Miriam, O'Dey Dan mon, Deumens Ronald, Lassner Franz, Schügner Frank, Gröger Andreas, Smeets Ralf, Brook Gary, Pallua Norbert
Department of Plastic, Reconstructive, and Hand Surgery, Burn Center, University Hospital RWTH Aachen, Pauwelsstrasse 30, Aachen, Germany.
J Neurosci Methods. 2008 May 15;170(1):117-22. doi: 10.1016/j.jneumeth.2008.01.006. Epub 2008 Jan 18.
Complete nerve transection (neurotmesis) of the rat sciatic nerve is a well-established animal model. The most frequently used behavioural for evaluation of neurotmesis-induced deficits is the walking track analysis with calculation of the sciatic functional index (SFI). More recently, the static sciatic index (SSI) has been developed, which shows a good correlation with the SFI. However, despite all advantages (high accessibility, easy handling, high accuracy, cost-effectiveness), the SSI is still not widely used. We, therefore, developed a novel programme ("Visual-SSI"), which will be made freely available for the assessment of the SSI. As gold-standard for the treatment of neurotmesis-induced nerve gaps, autologous nerve transplantation studies in the rat sciatic nerve model (n=16 [6 weeks], n=8 [12 weeks]) were carried out to test the effectiveness and feasibility of the Visual-SSI software. We observed a significant recovery starting from the pre-operative condition over the 3rd, 6th, 9th weeks until the 12th week after surgery (p<0.05). Theoretically, the SSI can be calculated from both rearing and normal standing position of the rats and we investigated whether the SSI is affected differentially by these positions. We observed no significant differences between animals in a rearing and normal standing stance (p>0.05). The present method combines efficiency (simplicity of use, rapid and economical setup) with accurate and precise quantification of the functional regeneration in the sciatic nerve lesion model of the rat.
大鼠坐骨神经完全横断（神经断伤）是一种成熟的动物模型。评估神经断伤所致功能缺陷最常用的行为学方法是行走轨迹分析，并计算坐骨神经功能指数（SFI）。最近，静态坐骨指数（SSI）已被开发出来，它与SFI具有良好的相关性。然而，尽管SSI具有所有优点（易于操作、易于处理、准确性高、成本效益高），但仍未得到广泛应用。因此，我们开发了一个新程序（“Visual-SSI”），该程序将免费提供用于评估SSI。作为治疗神经断伤所致神经缺损的金标准，在大鼠坐骨神经模型（n = 16 [6周]，n = 8 [12周]）中进行了自体神经移植研究，以测试Visual-SSI软件的有效性和可行性。我们观察到从术前状态到术后第3周、第6周、第9周直至第12周有显著恢复（p<0.05）。理论上，SSI可以从大鼠的站立和正常站立姿势计算得出，我们研究了这些姿势是否对SSI有不同影响。我们观察到站立和正常站立姿势的动物之间没有显著差异（p>0.05）。本方法将效率（使用简单、设置快速且经济）与大鼠坐骨神经损伤模型中功能再生的准确和精确量化相结合。
