Blaesen Markus, Friehs Karl, Flaschel Erwin
Bielefeld University, Faculty of Technology, Fermentation Engineering, D-33594 Bielefeld, Germany.
J Biotechnol. 2007 Dec 1;132(4):431-7. doi: 10.1016/j.jbiotec.2007.08.011. Epub 2007 Aug 10.
The Gram-negative bacterium Escherichia coli B-3996 represents an interesting host organism for the production of the essential amino acid L-threonine. Microbial processes - especially those of aerobic cultivation - lead to the generation of considerable amounts of biomass, thus lowering the product yield. These are the reasons for studying methods for the recycling of biomass from E. coli. It will be shown that it is possible to disintegrate the microbial biomass - preferably by means of high pressure homogenisation followed by a protease treatment of the resulting slurry of debris - in an efficient way and to recycle at least different amounts of the soluble part as cultivation medium component. By studying the growth and product formation of E. coli no adverse effects have been observed.
革兰氏阴性菌大肠杆菌B-3996是生产必需氨基酸L-苏氨酸的一种有趣的宿主生物。微生物过程——尤其是有氧培养过程——会产生大量生物质，从而降低产品产量。这些就是研究从大肠杆菌中回收生物质方法的原因。结果表明，有可能以高效的方式分解微生物生物质——最好是通过高压均质化，然后对所得碎片浆液进行蛋白酶处理——并将至少不同量的可溶部分作为培养基成分进行回收。通过研究大肠杆菌的生长和产物形成，未观察到不利影响。
