Schlabe Juergen, Johnen Christa, Schwartlander Ruth, Moser Viola, Hartmann Bernd, Gerlach Jörg C, Küntscher Markus V
Charité, Campus Virchow-Clinic, Department of Surgery, University Medicine, Berlin, Germany.
Burns. 2008 May;34(3):376-84. doi: 10.1016/j.burns.2007.04.005. Epub 2007 Sep 14.
Previous studies demonstrated, that cultured epithelial autografts (CEA) can be isolated and skin cell sprays can be produced for application on different types of wounds. The purpose of the present study was to determine which cell types can be isolated from the human scalp and whether these cells can be used for spray transplantation.
Outer root sheath cells (ORS), keratinocytes, melanocytes, dermal papilla cells (DP), and dermal sheath cells (DSC) were isolated from human scalp tissue. Isolated cells were characterized, expanded and sprayed in an in vitro model. Growth behaviour, morphology and cell counts were compared with non-sprayed cells.
With acceptable time, equipment and laboratory personnel a sufficient amount of keratinocytes, ORS, melanocytes, DP cells and DSC cells could be achieved. The cells are sufficient for application as a cell spray. Cells, positive for Integrin alpha6, Cytokeratin 19, CD73 and CD105 were identified within the cultures.
Human scalp is suitable to gain epidermal and dermal cells for the development of therapeutic cell spray transplantation. Further studies have to determine, whether these cells can be combined to produce wound specific skin substitutes.
先前的研究表明，可以分离培养的自体上皮移植片（CEA）并制备皮肤细胞喷雾，用于不同类型伤口的治疗。本研究的目的是确定可以从人类头皮中分离出哪些细胞类型，以及这些细胞是否可用于喷雾移植。
从人类头皮组织中分离外根鞘细胞（ORS）、角质形成细胞、黑素细胞、真皮乳头细胞（DP）和真皮鞘细胞（DSC）。对分离出的细胞进行表征、扩增，并在体外模型中进行喷雾处理。将生长行为、形态和细胞计数与未喷雾处理的细胞进行比较。
在可接受的时间、设备和实验室人员条件下，可以获得足够数量的角质形成细胞、ORS、黑素细胞、DP细胞和DSC细胞。这些细胞足以作为细胞喷雾使用。培养物中鉴定出整合素α6、细胞角蛋白19、CD73和CD105呈阳性的细胞。
人类头皮适合获取表皮和真皮细胞，用于开发治疗性细胞喷雾移植。进一步的研究必须确定这些细胞是否可以组合以生产针对伤口的皮肤替代物。
