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一种新型皮肤替代物的制备与表征

Preparation and characterization of a novel skin substitute.

作者信息

Castagnoli Carlotta, Fumagalli Mara, Alotto Daniela, Cambieri Irene, Casarin Stefania, Ostorero Alessia, Casimiri Raffaella, Germano Patrizia, Pezzuto Carla, Stella Maurizio

机构信息

Department of Plastic Surgery, Burn Unit and Skin Bank, Ospedale CTO Turin, Via Zuretti 29, Turin, Italy.

出版信息

J Biomed Biotechnol. 2010;2010. doi: 10.1155/2010/840363. Epub 2010 Sep 22.

DOI:10.1155/2010/840363
PMID:20936183
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2946634/
Abstract

Autologous epidermal cell cultures (CEA) represent a possibility to treat extensive burn lesions, since they allow a significative surface expansion which cannot be achieved with other surgical techniques based on autologous grafting. Moreover currently available CEA preparations are difficult to handle and their take rate is unpredictable. This study aimed at producing and evaluating a new cutaneous biosubstitute made up of alloplastic acellular glycerolized dermis (AAGD) and CEA to overcome these difficulties. A procedure that maintained an intact basement membrane was developed, so as to promote adhesion and growth of CEA on AAGD. Keratinocytes were seeded onto AAGD and cultured up to 21 days. Viability tests and immunohistochemical analysis with specific markers were carried out at 7, 14, and 21 days, to evaluate keratinocyte adhesion, growth, and maturation. Our results support the hypothesis that this newly formed skin substitute could allow its permanent engraftment in clinical application.

摘要

自体表皮细胞培养物（CEA）为治疗大面积烧伤创面提供了一种可能，因为它们能实现显著的表面扩张，这是其他基于自体移植的手术技术所无法达到的。此外，目前可用的CEA制剂难以操作，其成活率也难以预测。本研究旨在制备并评估一种由异体脱细胞甘油化真皮（AAGD）和CEA组成的新型皮肤生物替代物，以克服这些困难。我们开发了一种能维持完整基底膜的方法，以促进CEA在AAGD上的黏附和生长。将角质形成细胞接种到AAGD上并培养21天。在第7天、14天和21天进行活力测试和使用特异性标志物的免疫组织化学分析，以评估角质形成细胞的黏附、生长和成熟情况。我们的结果支持这样一种假设，即这种新形成的皮肤替代物在临床应用中能够实现永久移植。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/398185f21d82/JBB2010-840363.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/4d60a9ce3e4f/JBB2010-840363.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/28e08551298c/JBB2010-840363.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/9a2a8ad4ac7c/JBB2010-840363.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/c17c52981aa5/JBB2010-840363.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/a4e1052e0747/JBB2010-840363.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/a09ef21f6f9d/JBB2010-840363.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/ef40a8fcf74c/JBB2010-840363.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/b507acb5ad80/JBB2010-840363.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/db4c0a7c7964/JBB2010-840363.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/398185f21d82/JBB2010-840363.010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/4d60a9ce3e4f/JBB2010-840363.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/28e08551298c/JBB2010-840363.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/9a2a8ad4ac7c/JBB2010-840363.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/c17c52981aa5/JBB2010-840363.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/a4e1052e0747/JBB2010-840363.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/a09ef21f6f9d/JBB2010-840363.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/ef40a8fcf74c/JBB2010-840363.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/b507acb5ad80/JBB2010-840363.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/db4c0a7c7964/JBB2010-840363.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c506/2946634/398185f21d82/JBB2010-840363.010.jpg

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