Stettner Georg M, Zanella Sébastien, Huppke Peter, Gärtner Jutta, Hilaire Gérard, Dutschmann Mathias
Department of Pediatrics and Pediatric Neurology, University Medicine Göttingen, Georg August University, Robert-Koch-Str. 40, D-37075 Göttingen, Germany.
Respir Physiol Neurobiol. 2008 Jan 1;160(1):21-7. doi: 10.1016/j.resp.2007.07.011. Epub 2007 Aug 6.
Despite the clinical significance of central apneas in a wide range of disorders little is known about their pathogenesis. Research in this field has been hindered by the lack of appropriate animal models. Our goal was to determine whether the C57BL/6J mouse strain, which has an inherited predisposition for dysrhythmic breathing, exhibits spontaneous apneas. In vivo plethysmography of unanesthetized, unrestrained adult C57BL/6J mice revealed a regular occurrence of spontaneous apneas. In situ recordings from respiratory outputs (phrenic, vagal, hypoglossal nerves) in the working heart-brainstem preparation (WHBP) also showed spontaneous central apneas accompanied by laryngeal closure as indicated by tonic vagal postinspiratory activity and increase in subglottal pressure. The apneas were further characterized by a hypoglossal discharge with delayed onset compared to the tonic vagal postinspiratory activity. We conclude that spontaneous central apneas with active laryngeal closure occur in C57BL/6J mice. This mouse strain is a useful animal model to study neuronal mechanisms that underlie the generation of spontaneous central apneas.
尽管中枢性呼吸暂停在多种疾病中具有临床意义，但其发病机制仍知之甚少。该领域的研究因缺乏合适的动物模型而受到阻碍。我们的目标是确定具有遗传性心律失常呼吸倾向的C57BL/6J小鼠品系是否会出现自发性呼吸暂停。对未麻醉、不受约束的成年C57BL/6J小鼠进行的体内体积描记法显示，自发性呼吸暂停经常发生。在工作心脏-脑干标本（WHBP）中对呼吸输出（膈神经、迷走神经、舌下神经）进行的原位记录也显示，自发性中枢性呼吸暂停伴有喉部关闭，表现为迷走神经吸气后紧张性活动和声门下压力增加。与迷走神经吸气后紧张性活动相比，呼吸暂停的进一步特征是舌下神经放电延迟。我们得出结论，C57BL/6J小鼠会出现伴有主动喉部关闭的自发性中枢性呼吸暂停。该小鼠品系是研究自发性中枢性呼吸暂停产生的神经元机制的有用动物模型。
