Santoro Patricia Helena, Neves Pedro Manuel Oliveira Janeiro, Alexandre Talita Moretto, Sartori Daniele, Alves Luis Francisco Angeli, Fungaro Maria Helena Pelegrinelli
Universidade Estadual de Londrina, Centro de Ciências Agrárias Rodovia Celso Garcia Cid, CEP 86051-990 Londrina, PR, Brazil.
J Invertebr Pathol. 2008 Feb;97(2):83-90. doi: 10.1016/j.jip.2007.07.009. Epub 2007 Aug 6.
This paper presents results on isolates of the entomopathogenic fungus Beauveria bassiana for Alphitobius diaperinus (Coleoptera: Tenebrionidae) control. Of the 30 isolates tested, four (CG 71, CG 152, UNIOESTE 4, and UNIOESTE 40) resulted in mortality rates >or= 40% within 10 days. These mortality rates could be considered high because of the resistance of these species to B. bassiana. Tests were conducted using these isolates to estimate LC(50), mortality rate over time, vegetative growth, and conidial production on artificial medium and on insects. Isolates CG 71, CG 152, and UNIOESTE 4 showed the best performance and great potential to be used in an integrated management program in poultry farms to control A. diaperinus. Also, the molecular profiles of 12 isolates were analyzed using the RAPD technique. The high-virulence isolates presented a more homogeneous RAPD pattern than the others. Genetic sequencing of the ITS region was performed for one of the virulent isolates (UNIOESTE 4) and compared with sequences deposited at the NCBI database, confirming its taxonomical position as belonging to B. bassiana Clade A.
本文介绍了用于防治暗黑鳃金龟（鞘翅目：拟步甲科）的球孢白僵菌分离株的研究结果。在测试的30个分离株中，有4个（CG 71、CG 152、UNIOESTE 4和UNIOESTE 40）在10天内导致死亡率≥40%。鉴于这些物种对球孢白僵菌具有抗性，这些死亡率可被视为较高。使用这些分离株进行了测试，以估计半数致死浓度（LC50）、随时间变化的死亡率、营养生长以及在人工培养基和昆虫上的分生孢子产量。分离株CG 71、CG 152和UNIOESTE 4表现出最佳性能，在用于家禽养殖场综合管理计划以防治暗黑鳃金龟方面具有巨大潜力。此外，使用随机扩增多态性DNA（RAPD）技术分析了12个分离株的分子图谱。高毒力分离株呈现出比其他分离株更均匀的RAPD模式。对其中一个毒力分离株（UNIOESTE 4）进行了ITS区域的基因测序，并与NCBI数据库中保存的序列进行了比较，确认其分类地位属于球孢白僵菌A类群。
