Lee Kyung-Bok, Jung Young Hwan, Lee Zee-Won, Kim Soohyun, Choi Insung S
Glycomics Team, Korea Basic Science Institute, Daejeon 305-333, Republic of Korea.
Biomaterials. 2007 Dec;28(36):5594-600. doi: 10.1016/j.biomaterials.2007.08.036. Epub 2007 Sep 14.
In this paper, we report a simple method for spatially confining Bacillus subtilis (BS) spores into semi-three dimensional, non-biofouling microwells by using biospecific (such as biotin-streptavidin) interactions. Non-biofouling poly(ethylene glycol) (PEG)-based microwells were fabricated by employing a process of capillary molding on a glass slide. The biospecific interactions between biotinylated BS spores and streptavidin led to the selective deposition of BS spores onto the bottom of the microwells of which presented streptavidin. The viability of the patterned spores was confirmed by the induction of germination. Bacterial spores were found to maintain extreme robustness until they were exposed to favorable conditions. This work suggests that the use of bacterial spore-based sensors would increase the shelf-life (such as long-term storage and stability) of cell-based sensors.
在本文中，我们报告了一种简单的方法，即通过生物特异性（如生物素-链霉亲和素）相互作用将枯草芽孢杆菌（BS）孢子空间限制在半三维、无生物污染的微孔中。基于聚乙二醇（PEG）的无生物污染微孔通过在载玻片上采用毛细管成型工艺制备。生物素化的BS孢子与链霉亲和素之间的生物特异性相互作用导致BS孢子选择性沉积到呈现链霉亲和素的微孔底部。通过诱导萌发证实了图案化孢子的活力。发现细菌孢子在暴露于有利条件之前保持极强的稳健性。这项工作表明，使用基于细菌孢子的传感器将延长基于细胞的传感器的保质期（如长期储存和稳定性）。
