Park Tae Jung, Lee Kyung-Bok, Lee Seok Jae, Park Jong Pil, Lee Zee-Won, Choi Soo-Keun, Jung Heung-Chae, Pan Jae-Gu, Lee Sang Yup, Choi Insung S
Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, Korea.
J Am Chem Soc. 2004 Sep 1;126(34):10512-3. doi: 10.1021/ja047894y.
Micropatterns of Bacillus thuringiensis spores were generated by a combination of surface chemistry, microcontact printing, and spore surface display technique. The outermost layers of B. thuringiensis spores were engineered to present enhanced green fluorescent protein (EGFP), and the biospecific interaction between biotin and streptavidin was utilized to spatially direct the EGFP-presenting spores onto the micropatterned surfaces. The viability of the micropatterned spores was confirmed by the pattern generation of vegetative cells after the germination.
通过表面化学、微接触印刷和孢子表面展示技术相结合的方法，生成了苏云金芽孢杆菌孢子的微图案。对苏云金芽孢杆菌孢子的最外层进行改造，使其呈现增强型绿色荧光蛋白（EGFP），并利用生物素和链霉亲和素之间的生物特异性相互作用，将呈现EGFP的孢子在空间上定向到微图案化表面。通过发芽后营养细胞的图案生成，证实了微图案化孢子的活力。
