酪蛋白巨肽在双水相体系中的分配

Partitioning of caseinomacropeptide in aqueous two-phase systems.

作者信息

da Silva César A Sodré, Coimbra Jane S R, Rojas Edwin E Garcia, Minim Luis A, da Silva Luis Henrique Mendes

机构信息

Separation Process Laboratory (LPS), Department of Food Technology, Federal University of Viçosa (UFV), P. H. Rolfs Av., s/n, 36570-000 Viçosa, MG, Brazil.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Oct 15;858(1-2):205-10. doi: 10.1016/j.jchromb.2007.08.033. Epub 2007 Aug 31.

DOI:10.1016/j.jchromb.2007.08.033

PMID:17869592

Abstract

This study evaluates the influence of type of salt and temperature on the partition coefficient of caseinomacropetide (CMP) to determine the best conditions for the recovery of CMP in aqueous two-phase systems (ATPS) composed by poly(ethylene glycol) (PEG) 1500 and an inorganic salt (potassium phosphate, sodium citrate, lithium sulfate or sodium sulfate). In all systems, CMP presented affinity for the PEG-rich phase. The PEG1500+lithium sulfate showed the highest values of partitioning coefficient. In addition, thermodynamic parameters (DeltaH degrees , DeltaS degrees , DeltaG degrees) as a function of temperature, were calculated for the system PEG1500-sodium citrate at different PEG concentrations and the results imply thermodynamic differences between partitioning of CMP in this system.

摘要

本研究评估了盐的种类和温度对酪蛋白巨肽（CMP）分配系数的影响，以确定在由聚乙二醇（PEG）1500和无机盐（磷酸钾、柠檬酸钠、硫酸锂或硫酸钠）组成的双水相体系（ATPS）中回收CMP的最佳条件。在所有体系中，CMP对富含PEG的相具有亲和力。PEG1500 + 硫酸锂表现出最高的分配系数值。此外，针对不同PEG浓度的PEG1500 - 柠檬酸钠体系，计算了作为温度函数的热力学参数（ΔH°、ΔS°、ΔG°），结果表明该体系中CMP分配存在热力学差异。