Asami M, Owhashi M, Abe T, Nawa Y
Department of Parasitology, Miyazaki Medical College, Japan.
Immunol Cell Biol. 1991 Oct;69 ( Pt 5):355-60. doi: 10.1038/icb.1991.51.
The susceptibility of haemopoietic stem cell deficient W/Wv mice to infection with Plasmodium berghei was examined. The mean survival time of W/Wv mice after the infection was shorter than that of the +/+ mice. Splenomegaly, a characteristic pathological change of the host after infection with malaria parasites was not observed in W/Wv mice. When haemopoietic activity of the infected mice was examined, a substantial increase in number of multipotent haemopoietic stem cells (CFU-S) and the committed stem cells for granulocytes and macrophages (CFU-GM) or for erythrocytes (CFU-E) was observed in the bone marrow and spleen of +/+ but not of W/Wv mice. CFU-S were not detected in W/Wv mice before or after infection. The number of CFU-GM and CFU-E in bone marrow and spleen of W/Wv mice decreased after infection. Bone marrow grafting from +/+ to W/Wv mice 8 weeks before infection prolonged the mean survival time of the mice and effectively restored the number of CFU-S in the spleen of W/Wv mice. These results indicate that multi-potent haemopoietic stem cells play an important role in the host's defence mechanisms against P. berghei-infection.
研究了造血干细胞缺陷的W/Wv小鼠对伯氏疟原虫感染的易感性。感染后，W/Wv小鼠的平均存活时间比+/+小鼠短。W/Wv小鼠未出现脾肿大，而脾肿大是疟原虫感染后宿主的特征性病理变化。检查感染小鼠的造血活性时，在+/+小鼠的骨髓和脾脏中观察到多能造血干细胞（CFU-S）以及粒细胞和巨噬细胞定向干细胞（CFU-GM）或红细胞定向干细胞（CFU-E）数量大幅增加，而W/Wv小鼠则未出现这种情况。在感染前后的W/Wv小鼠中均未检测到CFU-S。感染后，W/Wv小鼠骨髓和脾脏中的CFU-GM和CFU-E数量减少。在感染前8周将+/+小鼠的骨髓移植到W/Wv小鼠体内，可延长小鼠的平均存活时间，并有效恢复W/Wv小鼠脾脏中CFU-S的数量。这些结果表明，多能造血干细胞在宿主抵御伯氏疟原虫感染的防御机制中发挥着重要作用。
