Xi Zhi-qin, Wang Lin-yuan, Sun Ji-jun, Liu Xian-zeng, Zhu Xi, Xiao Fei, Guan Li-feng, Li Jin-mei, Wang Liang, Wang Xue-feng
Department of Neurology, the First Affiliated Hospital, Chongqing Medical University, 1 You Yi Road, Chongqing 400016, China.
Neurosci Lett. 2007 Sep 20;425(1):53-8. doi: 10.1016/j.neulet.2007.08.016. Epub 2007 Aug 15.
TDAG51 (T cell death-associated gene 51) is an apoptosis-associated protein. Our aim was to investigate TDAG51 expression in the anterior temporal neocortex of patients with intractable epilepsy (IE), and then to discuss the possible role of TDAG51 in IE. Tissue samples from the anterior temporal neocortex of 33 patients who had surgery for IE were used to detect TDAG51 expression by immunohistochemistry, immunofluorescence, and Western blotting. We compared these tissues with nine histologically normal anterior temporal lobes from intracranial hypertension patients who had decompression procedures. TDAG51 was mainly expressed in the cytoplasm of neurons and glial cells. TDAG51 in IE was significantly higher than that in the controls. These findings were consistently observed using Western blotting, immunofluorescence, and immunohistochemistry techniques. TDAG51 in patients with IE was significantly higher when compared with levels in the controls. This finding suggests TDAG51 is consistent with a possible role of this gene in the evolution of the pathology in IE.
TDAG51（T细胞死亡相关基因51）是一种与细胞凋亡相关的蛋白质。我们的目的是研究TDAG51在难治性癫痫（IE）患者颞叶前部新皮质中的表达，进而探讨TDAG51在IE中的可能作用。采用免疫组织化学、免疫荧光和蛋白质免疫印迹法，检测33例接受IE手术患者颞叶前部新皮质组织样本中TDAG51的表达。我们将这些组织与9例因颅内高压接受减压手术患者的组织学正常的颞叶前部组织进行比较。TDAG51主要表达于神经元和神经胶质细胞的细胞质中。IE患者的TDAG51明显高于对照组。使用蛋白质免疫印迹法、免疫荧光法和免疫组织化学技术均一致观察到这些结果。与对照组相比，IE患者的TDAG51明显更高。这一发现表明TDAG51可能与该基因在IE病理演变中的作用相符。
