Pramyothin Pornpen, Ngamtin Chanon, Poungshompoo Somlak, Chaichantipyuth Chaiyo
Pharmacological Action of Natural Products Research Unit, Department of Pharmacology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
J Ethnopharmacol. 2007 Nov 1;114(2):169-73. doi: 10.1016/j.jep.2007.07.037. Epub 2007 Aug 11.
The present study was undertaken to investigate the protective effect and possible mechanism of aqueous extract from Phyllanthus amarus Schum. et. Thonn. (PA) on ethanol-induced rat hepatic injury. In the in vitro study, PA (1-4 mg/ml) increased %MTT reduction assay and decreased the release of transaminases (AST and ALT) in rat primary cultured hepatocytes being treated with ethanol. Hepatotoxic parameters studied in vivo included serum transaminases (AST and ALT), serum triglyceride (STG), hepatic triglyceride (HTG), tumor necrosis factor alpha (TNF-alpha), interleukin 1 beta (IL-1beta), together with histopathological examination. In acute toxicity study, single dose of PA (25, 50 and 75 mg/kg, p.o.) or SL (silymarin, a reference hepatoprotective agent, 5 mg/kg), 24h before ethanol (5 g/kg, p.o.) lowered the ethanol-induced levels of transaminases (AST and/or ALT). The 75 mg/kg PA dose gave the best result similar to SL. Treatment of rats with PA (75 mg/(kg day), p.o.) or SL (5 g/(kg day), p.o.) for 7 days after 21 days with ethanol (4 g/(kg day), p.o.) enhanced liver cell recovery by bringing the levels of AST, ALT, HTG and TNF-alpha back to normal. Histopathological observations confirmed the beneficial roles of PA and SL against ethanol-induced liver injury in rats. Possible mechanism may involve their antioxidant activity.
本研究旨在探讨苦味叶下珠水提取物对乙醇诱导的大鼠肝损伤的保护作用及其可能机制。在体外研究中，苦味叶下珠（1-4mg/ml）可提高大鼠原代培养肝细胞的MTT还原率，并降低乙醇处理组细胞的转氨酶（AST和ALT）释放。体内研究的肝毒性参数包括血清转氨酶（AST和ALT）、血清甘油三酯（STG）、肝甘油三酯（HTG）、肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β），并进行组织病理学检查。在急性毒性研究中，于乙醇（5g/kg，口服）给药前24小时，单次给予苦味叶下珠（25、50和75mg/kg，口服）或水飞蓟宾（一种保肝参考药物，5mg/kg），可降低乙醇诱导的转氨酶（AST和/或ALT）水平。75mg/kg的苦味叶下珠剂量效果最佳，与水飞蓟宾相似。在大鼠经乙醇（4g/(kg·天)，口服）处理21天后，给予苦味叶下珠（75mg/(kg·天)，口服）或水飞蓟宾（5mg/(kg·天)，口服），连续7天，可使AST、ALT、HTG和TNF-α水平恢复正常，促进肝细胞恢复。组织病理学观察证实了苦味叶下珠和水飞蓟宾对大鼠乙醇诱导肝损伤的有益作用。可能的机制可能涉及其抗氧化活性。
