Kogut M H, Eirmann L
Department of Biological Sciences, University of Arkansas, Fayetteville.
Int J Parasitol. 1991 Dec;21(8):979-83. doi: 10.1016/0020-7519(91)90177-9.
The role of T lymphocytes on the development of turkey coccidia in chickens was investigated to further document the involvement of immune mechanisms in the reactions of non-specific hosts to infections with species of Eimeria. All chickens treated with the T cell-specific immunosuppressive drug, cyclosporin A (CsA), 1, 2 or 3 days before or for 3 consecutive days immediately before inoculation with sporulated oocysts of Eimeria from the turkey, shed oocysts in their feces 7-12 days after inoculation. Those chickens treated with CsA after inoculation produced no oocysts although in one experiment a small number of oocysts were discharged by birds injected with CsA 1 day after inoculation. High serum levels of CsA were maintained in the CsA-treated chickens for at least 24 h post-inoculation with the oocysts. Immunosuppressive activity of the drug was determined by measuring lymphocyte blastogenesis in response to concanavalin A. These results demonstrate that the suppression of T lymphocyte activity in a non-specific host early during an infection with a heterologous species of Eimeria permits the complete intracellular development of the parasite. Our findings are suggestive of a role for T lymphocytes in preventing the development of Eimeria in non-specific hosts possibly via a lymphokine-mediated mechanism.
研究了T淋巴细胞在鸡体内火鸡球虫发育过程中的作用，以进一步证明免疫机制在非特异性宿主对艾美耳属物种感染反应中的参与情况。在用来自火鸡的艾美耳球虫卵囊接种前1天、2天或3天或紧接接种前连续3天，用T细胞特异性免疫抑制药物环孢素A（CsA）处理的所有鸡，在接种后7 - 12天粪便中排出卵囊。接种后用CsA处理的那些鸡没有产生卵囊，尽管在一项实验中，接种后1天注射CsA的鸡排出了少量卵囊。在用卵囊接种后，CsA处理的鸡中至少在24小时内维持了高血清水平的CsA。通过测量淋巴细胞对刀豆球蛋白A的增殖反应来确定该药物的免疫抑制活性。这些结果表明，在感染异源艾美耳属物种的早期，非特异性宿主中T淋巴细胞活性的抑制允许寄生虫在细胞内完全发育。我们的发现提示T淋巴细胞可能通过一种淋巴因子介导的机制在非特异性宿主中预防艾美耳球虫的发育中发挥作用。
