Gai S Annie, Wittrup K Dane
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Room E19-563, Cambridge, MA 02139, USA.
Curr Opin Struct Biol. 2007 Aug;17(4):467-73. doi: 10.1016/j.sbi.2007.08.012. Epub 2007 Sep 17.
Yeast surface display is being employed to engineer desirable properties into proteins for a broad variety of applications. Labeling with soluble ligands enables rapid and quantitative analysis of yeast-displayed libraries by flow cytometry, while cell-surface selections allow screening of libraries with insoluble or even as-yet-uncharacterized binding targets. In parallel, the utilization of yeast surface display for protein characterization, including in particular the mapping of functional epitopes mediating protein-protein interactions, represents a significant recent advance.
酵母表面展示技术正被用于改造蛋白质，使其具有理想特性，以适用于广泛的应用。用可溶性配体进行标记能够通过流式细胞术对酵母展示文库进行快速定量分析，而细胞表面筛选则允许筛选与不溶性甚至尚未表征的结合靶点的文库。与此同时，利用酵母表面展示技术进行蛋白质表征，特别是介导蛋白质-蛋白质相互作用的功能性表位图谱绘制，是近年来的一项重大进展。
