Lazarou Despoina, Grougnet Raphael, Papadopoulos Athanasios
Laboratory of Animal Physiology, School of Biology, Aristotle University, 54006, Thessaloniki, Greece.
Mutat Res. 2007 Dec 1;634(1-2):163-71. doi: 10.1016/j.mrgentox.2007.07.008. Epub 2007 Aug 3.
A polyphenolic mixture derived from sesame-seed perisperm (SSP) strongly reduced the mutagenicity of hydrogen peroxide (H(2)O(2)), sodium azide (NaN(3)), and benzo[a]pyrene (BaP) in strains TA100 and/or TA98 of Salmonella typhimurium. It exhibited desmutagenic activity against H(2)O(2), BaP in TA98 and/or TA100 and biomutagenic activity (apparently by affecting the DNA-repair system) against NaN(3) in strain TA100. According to in vitro experiments the polyphenolic mixture inhibited the activity of the CYP1A1 (EROD) enzyme responsible for the activation of BaP in the Ames' test, as well as that of the cytosolic enzyme GST. A cytosolic fraction from liver of male Wistar rats treated with either 20% SSP in the food, or 3mg or 6 mg of polyphenolic mixture/20 g food/day for a time period of 8 weeks reduced the mutagenic potential of BaP in strains TA100 and TA98, with the cytosolic fraction from rats treated with SSP causing the strongest reduction. Furthermore, a microsomal fraction from the 20% SSP-treated rats inhibited the mutagenicity of BaP in strains TA100 (26.3%) and TA98 (23%). In contrast, a microsomal fraction from rats treated with 3mg of polyphenolic mixture stimulated the mutagenicity of BaP in TA100 but reduced it in TA98, while for the microsomal fraction from rats treated with 6 mg of polyphenolic mixture, these effects on TA100 and TA98 were reversed.
一种源自芝麻种皮（SSP）的多酚混合物，能显著降低过氧化氢（H₂O₂）、叠氮化钠（NaN₃）和苯并[a]芘（BaP）对鼠伤寒沙门氏菌TA100和/或TA98菌株的致突变性。它对TA98和/或TA100菌株中的H₂O₂、BaP表现出抗诱变活性，对TA100菌株中的NaN₃表现出生物诱变活性（显然是通过影响DNA修复系统）。根据体外实验，该多酚混合物在艾姆斯试验中抑制了负责激活BaP的CYP1A1（EROD）酶以及胞质酶GST的活性。用食物中含20% SSP、或每天每20克食物含3毫克或6毫克多酚混合物处理雄性Wistar大鼠8周后，其肝脏胞质部分降低了BaP对TA100和TA98菌株的诱变潜力，其中用SSP处理的大鼠的胞质部分降低作用最强。此外，来自20% SSP处理大鼠的微粒体部分抑制了BaP对TA100（26.3%）和TA98（23%）菌株的致突变性。相比之下，用3毫克多酚混合物处理的大鼠的微粒体部分刺激了BaP对TA100的致突变性，但降低了对TA98的致突变性，而对于用6毫克多酚混合物处理的大鼠的微粒体部分，对TA100和TA98的这些影响则相反。
