Giard-Pasquier C, Rickenbacher U
Toxicology Department, Sandoz Pharma Ltd., Basle, Switzerland.
J Chromatogr. 1991 Aug 16;553(1-2):117-21. doi: 10.1016/s0021-9673(01)88479-x.
Prolactin and growth hormone are two peptide hormone with a very similar structure. A simple method is described for the simultaneous purification of these two peptides from canine pituitary extract by fast protein liquid chromatography. After extraction at pH 5.0 and 9.6 and anion-exchange chromatography on a MonoQ column, prolactin and growth hormone are then separated by gel filtration chromatography on two Superose columns coupled in series. The different fractions of the purification scheme are checked for the presence of the peptide hormones by sodium dodecyl sulphate gel electrophoresis in the Pharmacia PhastSystem. Each hormone is also characterized by its behaviour in a Western Blotting Detection System.
催乳素和生长激素是两种结构非常相似的肽类激素。本文描述了一种通过快速蛋白质液相色谱从犬垂体提取物中同时纯化这两种肽的简单方法。在pH 5.0和9.6条件下提取并在MonoQ柱上进行阴离子交换色谱后，催乳素和生长激素随后通过串联的两根Superose柱进行凝胶过滤色谱分离。在Pharmacia PhastSystem中通过十二烷基硫酸钠凝胶电泳检查纯化方案的不同级分中是否存在肽类激素。每种激素还通过其在蛋白质印迹检测系统中的行为进行表征。
