通过高效离子交换色谱法将牛催乳素纯化至均一状态。
Purification to homogeneity of bovine prolactin by high-performance ion-exchange chromatography.
作者信息
Berrini A, Borromeo V, Secchi C
机构信息
Istituto di Fisiologia Veterinaria e Biochimica, Università di Milano, Italy.
出版信息
J Chromatogr. 1991 Jun 28;547(1-2):457-61. doi: 10.1016/s0021-9673(01)88672-6.
Homogeneous bovine prolactin (bPRL) has been obtained using a procedure based on high-performance anion-exchange chromatography. The procedure enables up to 6 mg of 99.4% pure bPRL to be obtained per hour, with a recovery of 32.4%. The purity of the protein was checked by N-terminal sequencing and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The highly purified bPRL obtained with this method is suitable for complete structural and immunochemical studies.
采用基于高效阴离子交换色谱的方法已获得了均一的牛催乳素（bPRL）。该方法每小时能够获得高达6毫克纯度为99.4%的bPRL，回收率为32.4%。通过N端测序和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对蛋白质的纯度进行了检测。用这种方法获得的高度纯化的bPRL适用于完整的结构和免疫化学研究。
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