Van Vliet T, Van Schaik F, Van Schoonhoven J, Schrijver J
Department of Experimental Biology, TNO Toxicology and Nutrition Institute, Zeist, The Netherlands.
J Chromatogr. 1991 Aug 16;553(1-2):179-86. doi: 10.1016/s0021-9673(01)88486-7.
A method, using two different systems, is described for the high-performance liquid chromatographic analysis of retinol, retinal, retinoic acid, retinyl acetate, retinyl palmitate, alpha-, beta- and gamma-carotene, beta-apo-6'-, beta-apo-8', beta-apo-10'- and beta-apo-12'-carotenal, ethyl beta-apo-8'-carotenoate, alpha-tocopherol and alpha-tocopheryl acetate. The first system consists of a laboratory-packed Hypersil-ODS 3-microns column and a mobile phase of acetonitrile-methylene chloride-methanol-water (70:10:15:5, v/v). The second system consists of a laboratory-packed Hypersil-ODS 3-microns column and a mobile phase of acetonitrile-methylene chloride-methanol-water (70:10:15:5, v/v). The second system consists of a laboratory-packed Nucleosil C18 3-microns column and a mobile phase of acetonitrile-0.1 M ammonium acetate (80:20, v/v). The detection limits in standard solutions were 10 ng/ml for retinoids and carotenoids and 60 ng/ml for the E vitamers. Analysis of the tissues and plasma of rats, after 2 weeks on a diet supplemented with either beta-carotene or canthaxanthin (both 2 mg/g), led to the conclusion that the rats were able both to transport and store beta-carotene and canthaxanthin and to convert beta-carotene to retinol. Incubation of cytosol preparations from the mucosa of the small intestine of rat with 1 microgram of beta-carotene resulted in the formation of 10-20 ng of retinal within 1 h.
本文描述了一种使用两种不同系统的方法，用于高效液相色谱分析视黄醇、视黄醛、视黄酸、醋酸视黄酯、棕榈酸视黄酯、α-、β-和γ-胡萝卜素、β-阿朴-6'-、β-阿朴-8'、β-阿朴-10'-和β-阿朴-12'-胡萝卜醛、β-阿朴-8'-胡萝卜酸乙酯、α-生育酚和α-生育酚醋酸酯。第一个系统由实验室填充的3微米Hypersil-ODS柱和乙腈-二氯甲烷-甲醇-水（70:10:15:5，v/v）的流动相组成。第二个系统由实验室填充的3微米Hypersil-ODS柱和乙腈-二氯甲烷-甲醇-水（70:10:15:5，v/v）的流动相组成。第二个系统由实验室填充的3微米Nucleosil C18柱和乙腈-0.1M醋酸铵（80:20，v/v）的流动相组成。标准溶液中的检测限为类视黄醇和类胡萝卜素10 ng/ml，维生素E异构体60 ng/ml。对喂食β-胡萝卜素或角黄素（均为2 mg/g）2周后的大鼠组织和血浆进行分析，得出结论：大鼠既能转运和储存β-胡萝卜素和角黄素，又能将β-胡萝卜素转化为视黄醇。用1微克β-胡萝卜素孵育大鼠小肠黏膜的胞质溶胶制剂，1小时内可形成10-20 ng视黄醛。
