Melzi d'Eril G V, Rizzo V
Laboratorio di Analisi Chimico-Cliniche e Neurochimica, IRCCS, Fondazione 'Istituto Neurologico C. Mondino', Università di Pavia, Italy.
J Chromatogr. 1991 Aug 16;553(1-2):265-9. doi: 10.1016/s0021-9673(01)88497-1.
A procedure was devised for the determination of the unconjugated non-protein-bound fraction of 3,4-dihydroxyphenylalanine (DOPA) and 3-O-methyl-DOPA (3-OMD) in plasma using a reversed-phase liquid chromatographic system coupled with electrochemical detection. Sample preparation involves rapid isolation of the unbound drugs from the drug-protein complex by ultrafiltration through a membrane with a molecular weight cut-off of 10,000 dalton. One chromatographic run requires less than 10 min. The relative standard deviation is less than 3% for the within-assay imprecision and less than 4% for the between-assay imprecision. The detection limits for DOPA and 3-OMD are 0.2 and 1.3 ng/ml, respectively.
设计了一种使用反相液相色谱系统结合电化学检测来测定血浆中3,4-二羟基苯丙氨酸（DOPA）和3-O-甲基-DOPA（3-OMD）的未结合非蛋白结合部分的方法。样品制备包括通过分子量截留为10,000道尔顿的膜进行超滤，从药物-蛋白质复合物中快速分离出未结合的药物。一次色谱运行所需时间少于10分钟。批内不精密度的相对标准偏差小于3%，批间不精密度的相对标准偏差小于4%。DOPA和3-OMD的检测限分别为0.2和1.3 ng/ml。
