Ishimitsu T, Hirose S
J Chromatogr. 1985 Feb 8;337(2):239-48. doi: 10.1016/0378-4347(85)80037-2.
We devised a procedure for the simultaneous determination of 3,4-dihydroxyphenylalanine, catecholamines and O-methylated metabolites using a reversed-phase liquid chromatographic system. Detection is achieved by an electrochemical detector and a fluorescence detector connected in series. Sample preparation is kept to a minimum, and involves precipitation of proteins with trichloroacetic acid and perchloric acid, and subsequent neutralization, thus omitting the commonly adopted adsorption step. Chromatographic peaks were identified on the basis of retention behaviour and the ratio of responses at several oxidation potentials. The method was applied to the quantitative determination of 3,4-dihydroxyphenylalanine, catecholamines and O-methylated metabolites in human plasma.
我们设计了一种使用反相液相色谱系统同时测定3,4-二羟基苯丙氨酸、儿茶酚胺和O-甲基化代谢物的方法。检测通过串联的电化学检测器和荧光检测器实现。样品制备尽量简化，包括用三氯乙酸和高氯酸沉淀蛋白质，随后进行中和，从而省略了通常采用的吸附步骤。根据保留行为和几个氧化电位下的响应比来鉴定色谱峰。该方法应用于人体血浆中3,4-二羟基苯丙氨酸、儿茶酚胺和O-甲基化代谢物的定量测定。
