Yang X H, Wu Q L, Yu X B, Xu C X, Ma B F, Zhang X M, Li S N, Lahn B T, Xiang A P
Center for Stem Cell Biology and Tissue Engineering, SunYat-sen University, Guangzhou, Guangdong, China.
J Clin Pathol. 2008 Apr;61(4):467-73. doi: 10.1136/jcp.2007.047605. Epub 2007 Sep 14.
Nestin, an intermediate filament (IF) protein, is expressed in proliferating progenitor cells of developmental and regenerating tissues, and is identified as a neuroepithelial precursor cell marker. Recently, nestin was detected in some neoplasms such as glioma, ependymoma, melanoma, rhabdomyosarcoma, gastrointestinal stromal tumour (GIST), and testicular stromal tumour. Moreover, the expression intensity of nestin exhibited significant correlation with the malignant grade of glioma.
To detect the expression of nestin in different tumours and to analyse the relationship between the expression of nestin and the malignant grade of the tumours.
Formalin-fixed and paraffin-embedded surgical samples of neoplastic tissues were obtained from the Department of Pathology of Sun Yat-sen University. Histological analysis and immunohistochemical staining for nestin were performed. Histoscores were analysed by semi-quantitative evaluation.
Nestin was expressed predominantly in the cytoplasm of angiosarcoma, pancreatic adenocarcinoma and GIST samples, and some tumour cells expressed in the nucleus. There was a statistically significant difference between the histoscore of nestin in high malignant GIST (2.2366 (0.6920)) and that in low malignant GIST (1.3783 (0.4268)) (p = 0.003); and also between that in high malignant angiosarcoma (1.9188 (0.2069)) and that in low malignant angiosarcoma (0.6474 (0.3273)) (p = 0.000). Cavernous angioma did not express nestin. The histoscore of nestin in high malignant pancreatic adenocarcinoma (7/14) was 1.1767 (0.4676), and that in low malignant pancreatic adenocarcinoma (3/8) was 0.6577 (0.0056) (no significant difference, p = 0.112).
Results suggest that the expression of nestin may play an important role in the development of some neoplasms such as GIST and angiosarcoma.
巢蛋白是一种中间丝（IF）蛋白，在发育和再生组织的增殖祖细胞中表达，并被鉴定为神经上皮前体细胞标志物。最近，在一些肿瘤中检测到巢蛋白，如神经胶质瘤、室管膜瘤、黑色素瘤、横纹肌肉瘤、胃肠道间质瘤（GIST）和睾丸间质瘤。此外，巢蛋白的表达强度与神经胶质瘤的恶性分级显著相关。
检测巢蛋白在不同肿瘤中的表达，并分析巢蛋白表达与肿瘤恶性分级之间的关系。
从中山大学病理科获取福尔马林固定、石蜡包埋的肿瘤组织手术样本。进行组织学分析和巢蛋白免疫组化染色。通过半定量评估分析组织学评分。
巢蛋白主要在血管肉瘤、胰腺腺癌和GIST样本的细胞质中表达，一些肿瘤细胞在细胞核中表达。高恶性GIST中巢蛋白的组织学评分（2.2366（0.6920））与低恶性GIST中巢蛋白的组织学评分（1.3783（0.4268））之间存在统计学显著差异（p = 0.003）；高恶性血管肉瘤中巢蛋白的组织学评分（1.9188（0.2069））与低恶性血管肉瘤中巢蛋白的组织学评分（0.6474（0.3273））之间也存在统计学显著差异（p = 0.000）。海绵状血管瘤不表达巢蛋白。高恶性胰腺腺癌（7/14）中巢蛋白的组织学评分是1.1767（0.4676），低恶性胰腺腺癌（3/8）中巢蛋白的组织学评分是0.6577（0.0056）（无显著差异，p = 0.112）。
结果表明，巢蛋白的表达可能在一些肿瘤如GIST和血管肉瘤的发生发展中起重要作用。
