Shi Shuxian, Cheng Chun, Zhao Jian, Chen Mengling, Qin Jing, Gao Shangfeng, Shen Aiguo
The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, People's Republic of China.
J Mol Neurosci. 2007;32(1):64-71. doi: 10.1007/s12031-007-0015-6.
Skp2 (S-phase-associated kinase protein-2) is involved in ubiquitination and proteasome-mediated degradation of p27kip1, which plays important roles in cell cycle regulation and neurogenesis in the developing central nervous system (CNS). But their distribution and function in the nervous system lesion and regeneration remains unclear. In this study, we examined expression and relationship of p27kip1 and Skp2 in adult rat spinal cord following sciatic nerve injury. It was illustrated that they localized mainly in neurons and astrocytes of spinal cord, and might also expressed in other glial cells according to the results of immunohistochemistry. Sciatic nerve crush and transection resulted in a significant up-regulation of Skp2 and a down-regulation of p27kip1 in spinal cord. Statistical analysis indicated negative correlation between the number of p27kip1 and Skp2 positive cells in the ventral horn following the sciatic nerve lesion. Immunoprecipitation further showed that they interacted with each other in the regenerating process. Thus, p27kip1 and Skp2 likely play an important role in spinal cord regeneration after peripheral nerve injury.
Skp2（S期相关激酶蛋白2）参与p27kip1的泛素化及蛋白酶体介导的降解过程，p27kip1在发育中的中枢神经系统（CNS）的细胞周期调控和神经发生中起重要作用。但其在神经系统损伤和再生中的分布及功能仍不清楚。在本研究中，我们检测了坐骨神经损伤后成年大鼠脊髓中p27kip1和Skp2的表达及关系。免疫组化结果表明，它们主要定位于脊髓的神经元和星形胶质细胞，也可能在其他神经胶质细胞中表达。坐骨神经挤压和横断导致脊髓中Skp2显著上调，p27kip1下调。统计分析表明，坐骨神经损伤后腹角中p27kip1和Skp2阳性细胞数量呈负相关。免疫沉淀进一步表明，它们在再生过程中相互作用。因此，p27kip1和Skp2可能在周围神经损伤后的脊髓再生中起重要作用。
