Huang Ye, Liu Yonghua, Chen Ying, Yu Xiaowei, Yang Junling, Lu Mudan, Lu Qiuyan, Ke Qing, Shen Aiguo, Yan Meijuan
Orthopaedics of the Second Affiliated Hospital, Nanjing Medical University, Nanjing, 226001, China.
Cell Mol Neurobiol. 2009 May;29(3):403-11. doi: 10.1007/s10571-008-9332-8. Epub 2008 Dec 10.
Spy1, as a member of the Speedy/RINGO family and a novel activator of cyclin-dependent kinases, was shown to promote cell cycle progression and cell survival in response to DNA damage. While its expression and roles in nervous system lesion and repair were still unknown. Here, we performed an acute sciatic nerve injury model in adult rats and studied the dynamic changes of Spy1 expression in lumbar spinal cord. Temporally, Spy1 expression was increased shortly after sciatic nerve crush and peaked at day 2. Spatially, Spy1 was widely expressed in the lumbar spinal cord including neurons and glial cells. While after injury, Spy1 expression was increased predominantly in astrocytes and microglia, which were largely proliferated. Moreover, there was a concomitant up-regulation of CDK2 activity and down-regulation of p27. Collectively, we hypothesized peripheral nerve injury induced an up-regulation of Spy1 in lumbar spinal cord, which was associated with glial proliferation.
Spy1作为Speedy/RINGO家族的一员以及细胞周期蛋白依赖性激酶的新型激活剂，已被证明可促进细胞周期进程并在DNA损伤时促进细胞存活。而其在神经系统损伤和修复中的表达及作用仍不清楚。在此，我们在成年大鼠中建立了急性坐骨神经损伤模型，并研究了Spy1在腰脊髓中的动态表达变化。在时间上，坐骨神经挤压后不久Spy1表达即增加，并在第2天达到峰值。在空间上，Spy1在腰脊髓中广泛表达，包括神经元和神经胶质细胞。而损伤后，Spy1表达主要在大量增殖的星形胶质细胞和小胶质细胞中增加。此外，CDK2活性伴随上调，p27伴随下调。总体而言，我们推测外周神经损伤诱导腰脊髓中Spy1上调，这与神经胶质细胞增殖有关。
