Swoboda S, Brunner M, Boulyga S F, Galler P, Horacek M, Prohaska T
Department of Chemistry-VIRIS Project, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190, Vienna, Austria.
Anal Bioanal Chem. 2008 Jan;390(2):487-94. doi: 10.1007/s00216-007-1582-7. Epub 2007 Sep 14.
This work focuses on testing and application of Sr isotope signatures for the fast and reliable authentication and traceability of Asparagus officinalis originating from Marchfeld, Austria, using multicollector inductively coupled plasma mass spectrometry after optimised Rb/Sr separation. The major sample pool comprises freeze-dried and microwave-digested asparagus samples from Hungary and Slovakia which are compared with Austrian asparagus originating from the Marchfeld region, which is a protected geographical indication. Additional samples from Peru, The Netherlands and Germany were limited in number and allowed therefore only restricted statistical evaluation. Asparagus samples from Marchfeld were harvested within two subsequent years in order to investigate the annual variation. The results show that the Sr isotope ratio is consistent within these 2 years of investigation. Moreover, the Sr isotope ratio of total Sr in soil was found to be significantly higher than in an NH4NO3 extract, reflecting the mobile (bioavailable) phase. The isotope composition in the latter extract corresponds well to the range found in the asparagus samples in Marchfeld, even though the concentration of Sr in asparagus shows no direct correlation to the concentration of Sr in the mobile phase of the soil. The major question was whether the 'Marchfelder Spargel' can be distinguished from samples from the neighbouring countries of Hungary and Slovakia. According to our findings, they can be clearly (100%) singled out from the Hungarian samples and can be distinguished from the Slovakian asparagus samples with a probability of more than 80%.
这项工作聚焦于利用优化后的铷/锶分离法，通过多接收电感耦合等离子体质谱法，对源自奥地利马尔希费尔德的芦笋进行快速可靠的鉴定和溯源，测试并应用锶同位素特征。主要样本库包括来自匈牙利和斯洛伐克的冻干及微波消解芦笋样本，将其与源自马尔希费尔德地区的奥地利芦笋进行比较，该地区芦笋具有受保护的地理标志。来自秘鲁、荷兰和德国的额外样本数量有限，因此仅允许进行有限的统计评估。采集了马尔希费尔德两年内收获的芦笋样本，以研究年度变化。结果表明，在这两年的调查中，锶同位素比率是一致的。此外，发现土壤中总锶的同位素比率显著高于硝酸铵提取物中的比率，反映了可移动（生物可利用）相。尽管芦笋中锶的浓度与土壤可移动相中锶的浓度没有直接关联，但后者提取物中的同位素组成与马尔希费尔德芦笋样本中的范围非常吻合。主要问题是“马尔希费尔德芦笋”能否与来自匈牙利和斯洛伐克等邻国的样本区分开来。根据我们的研究结果，它们可以从匈牙利样本中清晰地（100%）区分出来，并且与斯洛伐克芦笋样本区分开来的概率超过80%。
