Failloux A B, Chanteau S, Chungue E, Loncke S, Sechan Y
Institut Territorial de Recherches Médicales Louis Malardé, Tahiti, Polynésie française.
J Am Mosq Control Assoc. 1991 Dec;7(4):660-2.
In order to construct a cDNA library from third-stage larvae (L3) of Wuchereria bancrofti var. pacifica, the Parafilm membrane feeding method is proposed for the oral infection of Aedes polynesiensis. Heparinized blood supplemented with 5.10(-3) M ATP was put in the feeder with carbon dioxide provided as additional phagostimulant. The results of this artificial infection feeding method were compared with those obtained when mosquitoes fed directly on the forearm of a microfilaremic patient. The number of females feeding through the artificial membrane was smaller than on the patient's forearm (32.1 vs. 84.8%). The mean number of L3s obtained per female was not statistically different between the 2 feeding methods; however, the total number of L3s obtained from 100 females allowed to feed in each group was twice as high in the natural feeding method.
为了构建太平洋班氏吴策线虫三期幼虫（L3）的cDNA文库，提出使用Parafilm膜饲喂法经口感染波利尼西亚伊蚊。将补充有5×10⁻³ M ATP的肝素化血液放入饲喂器中，并提供二氧化碳作为额外的摄食刺激剂。将这种人工感染饲喂方法的结果与蚊子直接吸食微丝蚴血症患者前臂血液时获得的结果进行比较。通过人工膜进食的雌蚊数量少于吸食患者前臂血液的雌蚊数量（32.1% 对84.8%）。两种饲喂方法中每只雌蚊获得的L3平均数量没有统计学差异；然而，每组100只允许进食的雌蚊所获得的L3总数在自然饲喂法中是人工饲喂法的两倍。
