Naito K, Toyoda Y
Institute of Medical Science, University of Tokyo, Japan.
J Reprod Fertil. 1991 Nov;93(2):467-73. doi: 10.1530/jrf.0.0930467.
Porcine oocytes cultured in follicular fluid for various periods of up to 48 h were stained with Hoechst-33342 and classified according to maturation before assaying. Histone H1 kinase activity at metaphase I was approximately 10 times that at the germinal vesicle stage. An abrupt reduction in activity was observed in oocytes emitting the first polar body; then the activity increased again to the same level as at metaphase I. This pattern is similar to those reported in non-mammalian species and supports the concepts that histone H1 kinase is ubiquitous in eukaryotes and controls the meiotic cell cycle in mammals.
将在卵泡液中培养长达48小时不同时间段的猪卵母细胞用Hoechst-33342染色，并在测定前根据成熟度进行分类。中期I时组蛋白H1激酶活性约为生发泡期的10倍。在排出第一极体的卵母细胞中观察到活性突然降低；然后活性再次升高至与中期I相同的水平。这种模式与非哺乳动物物种中报道的模式相似，并支持组蛋白H1激酶在真核生物中普遍存在并控制哺乳动物减数分裂细胞周期的概念。
