基于逆转录聚合酶链反应的口腔组织样本细胞色素P450表达谱
RT-PCR-based cytochrome P450 expression profile of oral tissue samples.
作者信息
Sarikaya D, Chiba I, Bilgen C, Kamatakii T, Topcu Z
机构信息
Department of Bioengineering, Faculty of Engineering, University of Hokkaido, Japan.
出版信息
J Clin Pharm Ther. 2007 Oct;32(5):445-8. doi: 10.1111/j.1365-2710.2007.00841.x.
OBJECTIVE
To analyse the expression of individual forms of cytochrome P450 (CYP) at the mRNA level in five homogenized oral buccal tissue samples from four individuals with or without oral malignancy.
METHOD
Individual forms of CYPs were studied by reverse transcriptase-polymerase chain reaction (RT-PCR), using specific primers for CYPs 2B6, 2C, 2D6, 2E1, 3A3/4 and 3A5, and oral CYP expressions were compared with CYP expression in liver tissue.
RESULTS
Consistent expression of CYPs 2C, 2E1 and 3A5 was observed in oral buccal tissue at mRNA level.
CONCLUSIONS
These particular CYPs have possible roles in the protection of the body against orally ingested xenobiotics as well as influence the bioavailability of therapeutic compounds.
目的
分析来自四名有或无口腔恶性肿瘤个体的五个口腔颊部组织匀浆样本中细胞色素P450（CYP）各亚型在mRNA水平的表达情况。
方法
采用逆转录聚合酶链反应（RT-PCR），使用针对CYP 2B6、2C、2D6、2E1、3A3/4和3A5的特异性引物研究CYP各亚型，并将口腔CYP表达与肝组织中的CYP表达进行比较。
结果
在口腔颊部组织的mRNA水平观察到CYP 2C、2E1和3A5的一致表达。
结论
这些特定的CYP在保护机体免受口服摄入的外源性物质影响以及影响治疗性化合物的生物利用度方面可能发挥作用。
Zotero 插件