异生物质代谢性细胞色素P450同工型在成人及胎儿肝脏中的表达
Expression of xenobiotic-metabolizing cytochrome P450 forms in human adult and fetal liver.
作者信息
Hakkola J, Pasanen M, Purkunen R, Saarikoski S, Pelkonen O, Mäenpää J, Rane A, Raunio H
机构信息
Department of Pharmacology and Toxicology, University of Oulu, Finland.
出版信息
Biochem Pharmacol. 1994 Jul 5;48(1):59-64. doi: 10.1016/0006-2952(94)90223-2.
Expression of human cytochrome P450 (CYP) genes in human adult and fetal liver were studied using the reverse transcriptase-polymerase chain reaction (RT-PCR) method. In adult liver mRNA of CYPs 1A1, 1A2, 2A6/2A7, 2B6/2B7, 2C8-19, 2D6, 2E1, 3A3/3A4 and 3A7 were detected while CYPs 2F1 and 4B1 were absent. In fetal liver mRNA of CYPs 2C8, 2D6, 3A3/3A4 and 3A7 were found but all other forms studied were undetectable. The results provide a comprehensive qualitative picture of the expression of CYP genes in families CYP1 through CYP4 in human adult and fetal liver.
采用逆转录-聚合酶链反应（RT-PCR）方法研究了人类细胞色素P450（CYP）基因在成人和胎儿肝脏中的表达情况。在成人肝脏中，检测到CYP 1A1、1A2、2A6/2A7、2B6/2B7、2C8 - 19、2D6、2E1、3A3/3A4和3A7的mRNA，而未检测到CYP 2F1和4B1。在胎儿肝脏中，发现了CYP 2C8、2D6、3A3/3A4和3A7的mRNA，但所研究的其他所有形式均未检测到。这些结果提供了人类成人和胎儿肝脏中CYP1至CYP4家族CYP基因表达的全面定性情况。
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