Zhang Qian, Zhang Ling, Liu Bin, Lu Xianbo, Li Jinghong
Department of Chemistry, University of Science and Technology of China, Hefei, China.
Biosens Bioelectron. 2007 Dec 15;23(5):695-700. doi: 10.1016/j.bios.2007.08.008. Epub 2007 Aug 15.
An organized multi-components hybrid material, constructed by mesopores cellular foam silicate (MCFs) and quantum dots (QDs), was designed for the immobilization and biosensing of protein. The negative CdTe QDs were assembled on the surface of mesopores in amino group functionalized MCFs through electrostatic interaction to form QDs-MCFs hybrid material, which was used as the matrix to immobilize myoglobin (Mb) and fabricate modified protein electrode (Mb-QDs-MCFs/GC). FT-IR, UV-vis and PL spectroscopies were used to monitor the assembly process and also demonstrated that Mb was immobilized into the hybrid matrix without denaturation. Compared with the Mb-MCFs/GC electrode, the Mb-QDs-MCFs/GC electrode could not only realize enhanced direct electrochemistry but also display better sensitivity and wider linear range to the detection of hydrogen peroxide. The experiment results demonstrate that the hybrid matrix provides a biocompatible microenvironment for protein and supplies a necessary pathway for its direct electron transfer.
设计了一种由介孔蜂窝泡沫硅酸盐（MCFs）和量子点（QDs）构建的有序多组分混合材料，用于蛋白质的固定化和生物传感。通过静电相互作用将负电荷的碲化镉量子点组装在氨基功能化MCFs的介孔表面，形成量子点-MCFs混合材料，用作固定肌红蛋白（Mb）和制备修饰蛋白电极（Mb-QDs-MCFs/GC）的基质。利用傅里叶变换红外光谱（FT-IR）、紫外可见光谱（UV-vis）和光致发光光谱（PL）监测组装过程，并证明Mb被固定在混合基质中且未发生变性。与Mb-MCFs/GC电极相比，Mb-QDs-MCFs/GC电极不仅能实现增强的直接电化学，而且对过氧化氢的检测具有更好的灵敏度和更宽的线性范围。实验结果表明，混合基质为蛋白质提供了生物相容性微环境，并为其直接电子转移提供了必要途径。
