Rathod Dinesh A, Kaur Amarjeet, Patel Vinod, Patel Kunjal, Kabrawala Raviraj, Patel Viral, Patel Mohak, Shah Pranay
Departments of Pathology, Smt NHL Municipal Medical College, Ahmedabad, India.
Am J Clin Pathol. 2007 Oct;128(4):585-9. doi: 10.1309/R1YL4B4BT2WCQDGV.
The present study aimed to retrospectively evaluate the usefulness of cell counter-based parameters and formulas in beta-thalassemia trait (BTT) detection. The study included 170 BTT cases (hemoglobin [Hb]A(2) >4.0% [0.04]) and 30 non-BTT cases (HbA(2), 2.3%-3.5% [0.02-0.04]). Depending on the hemoglobin level and iron deficiency, the BTT group was further classified into classic BTT (n = 112) and BTT with iron deficiency anemia (n = 58). The RBC count, MCH, MCV, RDW, and Shine and Lal, Mentzler, Srivastava, England and Fraser, Ricerca, and Green indexes were applied. For the first time in the population of India, these 10 cell counter parameters and manual formulas were compared with high-performance liquid chromatography-derived HbA2 levels for deriving a cost-effective alternative method; and receiver operating characteristic curves were applied. We found that the Shine and Lal, Srivastava, and Mentzler indexes, MCV, and MCH have better discriminative function than the RBC count and red cell distribution width and their related formulas.
本研究旨在回顾性评估基于血细胞计数器的参数和公式在β地中海贫血特征（BTT）检测中的实用性。该研究纳入了170例BTT病例（血红蛋白[Hb]A2>4.0%[0.04]）和30例非BTT病例（HbA2，2.3%-3.5%[0.02-0.04]）。根据血红蛋白水平和缺铁情况，BTT组进一步分为经典BTT（n=112）和缺铁性贫血BTT（n=58）。应用了红细胞计数、平均红细胞血红蛋白含量（MCH）、平均红细胞体积（MCV）、红细胞分布宽度（RDW）以及Shine和Lal、Mentzler、Srivastava、England和Fraser、Ricerca和Green指数。在印度人群中，首次将这10个血细胞计数器参数和手工公式与高效液相色谱法测定的HbA2水平进行比较，以得出一种经济有效的替代方法；并应用了受试者工作特征曲线。我们发现，Shine和Lal、Srivastava和Mentzler指数、MCV和MCH比红细胞计数和红细胞分布宽度及其相关公式具有更好的鉴别功能。
