Celli Susanna, Bousso Philippe
Départment d'Immunologie, Institut Pasteur, Paris, France.
Methods Mol Biol. 2007;380:355-63. doi: 10.1007/978-1-59745-395-0_22.
Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of lymph node T cells during either priming or tolerance, in live, anesthetized mice. Intravital imaging of T lymphocytes is a powerful tool to study the cellular orchestration of adaptive immune responses in physiological settings. This method should provide new insights into the regulation of lymphocyte migration and cell-cell interactions in various immunological contexts.
双光子显微镜能够对位于深部组织环境中的荧光标记细胞进行实时成像。我们描述了一种在活体麻醉小鼠中观察淋巴结T细胞在启动或耐受过程中行为的方法。T淋巴细胞的活体成像技术是研究生理环境下适应性免疫反应细胞调控的有力工具。该方法将为深入了解不同免疫背景下淋巴细胞迁移及细胞间相互作用的调控机制提供新的思路。
