淋巴结中自然杀伤细胞和树突状细胞的活体双光子成像。

Intravital two-photon imaging of natural killer cells and dendritic cells in lymph nodes.

作者信息

Celli Susanna, Breart Béatrice, Bousso Philippe

机构信息

Département d'Immunologie, G5 Dynamiques des Réponses Immunes, Equipe Avenir, Institut Pasteur, Paris Cedex, France.

出版信息

Methods Mol Biol. 2008;415:119-26. doi: 10.1007/978-1-59745-570-1_7.

DOI:10.1007/978-1-59745-570-1_7

PMID:18370151

Abstract

Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of natural killer (NK) cells and dendritic cells (DC) in the lymph nodes of live, anesthetized mice. Intravital two-photon imaging is a powerful tool to study the migration and cell interactions of immune cells such as NK and DC in physiological settings.

摘要

双光子显微镜使对位于深部组织环境中的荧光标记细胞进行实时成像成为可能。我们描述了一种在活体、麻醉小鼠的淋巴结中可视化自然杀伤（NK）细胞和树突状细胞（DC）行为的方法。活体双光子成像对于研究免疫细胞（如NK细胞和DC细胞）在生理环境中的迁移和细胞间相互作用而言是一种强大的工具。