Cheng Lu, Chong Mei, Fan Weiwei, Guo Xiaofang, Zhang Wenjuan, Yang Xueyan, Liu Fan, Gui Yonghao, Lu Daru
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, 200433, People's Republic of China.
Dev Genes Evol. 2007 Oct;217(10):699-707. doi: 10.1007/s00427-007-0177-9. Epub 2007 Sep 18.
The forkhead transcription factor gene family encodes a large group of proteins that play critical roles in many developmental events. In this report, we describe the isolation and characterization of foxp1 in zebrafish. The full-length zebrafish foxp1 cDNA contains 2,440 bp encoding a protein of 659 amino acids which shares 72, 68, 68, 70, 65, and 62% overall identity with human, mouse, rat, chicken, zebra finch, and frog Foxp1, respectively. Results of whole-mount in situ hybridization showed that foxp1 exhibits very complex and dynamic expression pattern during early embryonic development. Prominent foxp1 expression is detected in many regions of the developing central nervous system, especially in midbrain-hindbrain boundary, hindbrain, and spinal cord. Strong expression is also observed in retina, ear, branchial arches, hatching gland, heart, pronephric duct, gut, proctodeum, pectoral fin, and swim bladder. These results provide evidence that foxp1 is likely to function as a very important transcription factor in the development of the central nervous system and many other organs in zebrafish.
叉头转录因子基因家族编码一大类在许多发育事件中起关键作用的蛋白质。在本报告中，我们描述了斑马鱼中foxp1的分离和特征。斑马鱼foxp1全长cDNA包含2440个碱基对，编码一个659个氨基酸的蛋白质，该蛋白质与人类、小鼠、大鼠、鸡、斑胸草雀和青蛙的Foxp1的总体一致性分别为72%、68%、68%、70%、65%和62%。整体原位杂交结果表明，foxp1在胚胎早期发育过程中表现出非常复杂和动态的表达模式。在发育中的中枢神经系统的许多区域检测到显著的foxp1表达，特别是在中脑-后脑边界、后脑和脊髓。在视网膜、耳朵、鳃弓、孵化腺、心脏、前肾管、肠道、泄殖腔、胸鳍和鳔中也观察到强烈表达。这些结果提供了证据，表明foxp1可能在斑马鱼中枢神经系统和许多其他器官的发育中作为一个非常重要的转录因子发挥作用。
