Madhusudan G P, Dev R, Sharma M K, Singh Dheer
Molecular Endocrinology Laboratory, Division of Animal Biochemistry, National Dairy Research Institute, Karnal 132 001, India.
Indian J Exp Biol. 2007 Aug;45(8):669-75.
The tumor necrosis factor-alpha (TNF-alpha) plays an important role in ovarian follicular development and ovulation process and acts through its receptor (TNFRI). The present investigation describes the expression of mRNAs encoding TNF-alpha and TNFRI in relation to glyceraldehyde-3-phosphate dehydrogenase (G3PDH) and beta-actin as control genes, using RT-PCR, in granulosa cells, intact follicles and luteal tissues from buffalo ovary. There was significant higher expression of mRNAs encoding TNF-alpha in granulosa cells from medium follicles and TNFRI expression increased with increase in size of follicles. Post-ovulatory structures (corpus luteum and corpus albicans) exhibited significantly higher expression of TNFRI mRNAs as compared to that obtained in intact follicles suggesting its immediate and critical role just after ovulation, for mediating TNF-alpha action on these tissues. Though the expression of TNF-alpha mRNA was stimulated by treatment of granulosa cells with FSH during culture, the expression of TNFRI mRNA did not change. The FSH alongwith IGF-I did not exert any effect. These results suggested an important role of TNF-alpha and its receptor in buffalo ovarian functions.
肿瘤坏死因子-α（TNF-α）在卵泡发育和排卵过程中起重要作用，并通过其受体（TNFRI）发挥作用。本研究利用逆转录聚合酶链反应（RT-PCR），以甘油醛-3-磷酸脱氢酶（G3PDH）和β-肌动蛋白作为对照基因，描述了水牛卵巢颗粒细胞、完整卵泡和黄体组织中编码TNF-α和TNFRI的mRNA的表达情况。中等卵泡的颗粒细胞中编码TNF-α的mRNA表达显著更高，且TNFRI表达随卵泡大小增加而升高。与完整卵泡相比，排卵后结构（黄体和白体）中TNFRI mRNA的表达显著更高，这表明其在排卵后立即发挥关键作用，介导TNF-α对这些组织的作用。尽管在培养过程中用促卵泡激素（FSH）处理颗粒细胞可刺激TNF-α mRNA的表达，但TNFRI mRNA的表达并未改变。FSH与胰岛素样生长因子-I（IGF-I）共同作用也未产生任何影响。这些结果表明TNF-α及其受体在水牛卵巢功能中起重要作用。
