Debnath Siddhartha, Guha Debjani
S N Pradhan Centre for Neurosciences, University of Calcutta, 244 B A.J.C. Bose Road, Kolkata 700 020.
Indian J Exp Biol. 2007 Aug;45(8):726-31.
The present study has been undertaken to observe the effect of aqueous extract of M. oleifera (MO) leaf (300mg/kg body weight) on mean ulcer index, enterochromaffin (EC) cells and serotonin (5-hydroxytryptamine; 5-HT) content of ulcerated gastric tissue. Ulceration was induced by using aspirin (500 mg/kg, po), cerebellar nodular lesion and applying cold stress. In all cases increased mean ulcer index in gastric tissue along with decreased EC cell count was observed with concomitant decrease of 5-HT content. Pretreatment with MO for 14 days decreased mean ulcer index, increased both EC cell count and 5-HT content in all ulcerated group, but treatment with ondansetron, a 5-HT3 receptor antagonist, along with MO pretreatment increased mean ulcer index, decreased 5-HT content without any alteration in EC cell count. The results suggest that the protective effect of MO on ulceration is mediated by increased EC cell count and 5-HT levels which may act via 5-HT3 receptors on gastric tissue.
本研究旨在观察辣木（MO）叶水提取物（300mg/kg体重）对溃疡胃组织平均溃疡指数、肠嗜铬（EC）细胞及血清素（5-羟色胺；5-HT）含量的影响。通过使用阿司匹林（500mg/kg，口服）、小脑结节病变及施加冷应激诱导溃疡形成。在所有病例中，均观察到胃组织平均溃疡指数增加，同时EC细胞计数减少，伴随5-HT含量降低。用MO预处理14天可降低所有溃疡组的平均溃疡指数，增加EC细胞计数及5-HT含量，但5-HT3受体拮抗剂昂丹司琼与MO预处理联合使用时，平均溃疡指数增加，5-HT含量降低，而EC细胞计数无任何改变。结果表明，MO对溃疡的保护作用是通过增加EC细胞计数及5-HT水平介导的，这可能通过胃组织上的5-HT3受体发挥作用。
