辣木根和柑橘果皮提取物在治疗溃疡性结肠炎小鼠中的潜力。

Potential of Moringa oleifera root and Citrus sinensis fruit rind extracts in the treatment of ulcerative colitis in mice.

机构信息

Department of Pharmacognosy, Pravara Rural College of Pharmacy, Loni, Maharashtra, India.

出版信息

Pharm Biol. 2012 Oct;50(10):1297-302. doi: 10.3109/13880209.2012.674142. Epub 2012 Jul 31.

DOI:10.3109/13880209.2012.674142

PMID:22849565

Abstract

CONTEXT

The plant Moringa oleifera Lam (Moringaceae), commonly known as the drumstick tree, is an indigenous species in India. This species has been of interest to researchers because traditionally its roots are reported in the treatment of ulcerative colitis (UC). Traditionally it is reported that Citrus sinensis Linn (Rutaceae) fruit rind when combined with M. oleifera will increase the efficacy of the plant in the treatment of UC.

OBJECTIVE

The present work was undertaken to determine the effectiveness of M. oleifera root alone and in combination with C. sinensis fruit rind in the treatment of UC.

MATERIALS AND METHODS

Ethanol and aqueous extracts of M. oleifera roots (100 and 200 mg/kg, body weight) were screened alone and in equal combination with ethanol extract of C. sinensis fruit rind, i.e., 50 mg/kg each of C. sinensis and M. oleifera for their activity on acetic acid-induced UC in mice.

RESULTS

Treatment with combination of extracts of M. oleifera root and C. sinensis fruit rind (50 mg/kg, each) showed less ulceration and hyperemia than individual extract (200 mg/kg) in histopathological observation. Acetic acid increased myeloperoxidase (MPO) level in blood and colon tissue to 342 U/mL and 384 U/mg, respectively. Combination of ethanol extract of M. oleifera root with C. sinensis fruit rind extract significantly (p<0.05) decreased MPO in blood and tissue to 278 U/mL and 291 U/mg, respectively. MPO in blood and tissue in control group was 85 ± 1.2 U/mL and 96 ± 1.3 U/mg, respectively. Similarly this combination significantly reduced malondialdehyde (MDA) level in blood and tissue to 7.11 nmol/mL and 8.19 nmol/mg, from 11.20 nmol/mL and 13.20 nmol/mg, respectively. MDA in blood and tissue in control group was 2.76 ± 1.2 nmol/mL and 3.76 ± 1.2 nmol/mg, respectively.

DISCUSSION AND CONCLUSION

Results show that a combination of M. oleifera root extracts with C. sinensis fruit rind extract is effective in the treatment of UC and results are comparable with the standard drug prednisolone.

摘要

背景

辣木树（辣木科），通常被称为鼓槌树，是印度的一种本土物种。由于传统上报道其根部可用于治疗溃疡性结肠炎（UC），因此该物种一直引起研究人员的兴趣。传统上，有报道称，当与辣木树的根结合使用时，柑橘属柠檬（芸香科）的果皮会增加该植物治疗 UC 的功效。

目的

本研究旨在确定单独使用辣木树根和与柑橘属柠檬的果皮联合使用在治疗 UC 方面的有效性。

材料和方法

单独筛选辣木树根的乙醇和水提取物（100 和 200mg/kg，体重）以及柑橘属柠檬果皮的乙醇提取物（各 50mg/kg），以评估它们对乙酸诱导的 UC 小鼠的活性。

结果

与单独使用（200mg/kg）提取物相比，辣木树根和柑橘属柠檬果皮提取物（各 50mg/kg）联合使用在组织病理学观察中显示出较少的溃疡和充血。乙酸将血液和结肠组织中的髓过氧化物酶（MPO）水平分别增加到 342U/mL 和 384U/mg。辣木树根的乙醇提取物与柑橘属柠檬果皮提取物联合使用可显著（p<0.05）降低血液和组织中的 MPO 分别至 278U/mL 和 291U/mg。对照组血液和组织中的 MPO 分别为 85±1.2U/mL 和 96±1.3U/mg。同样，这种联合使用还显著降低了血液和组织中的丙二醛（MDA）水平，分别从 11.20nmol/mL 和 13.20nmol/mg 降至 7.11nmol/mL 和 8.19nmol/mg。对照组血液和组织中的 MDA 分别为 2.76±1.2nmol/mL 和 3.76±1.2nmol/mg。