Liu Chun-Yan, Liu Yu-Feng, Zeng Li, Zhang Shu-Guang, Xu Hui
Department of Pediatrics, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China.
Zhonghua Xue Ye Xue Za Zhi. 2007 Apr;28(4):255-8.
To investigate the mRNA expression of transferrin receptor 1 (TfR1) and iron regulatory protein 1 (IRP1) in the full-term placenta from different maternal iron status, and explore the mechanism of placental iron transport and regulation.
The mRNA level of TfR1 and IRP1 in full-term placentae was detected by reverse transcription polymerase chain reaction (RT-PCR) in normal group (N), iron deficiency group (ID) and iron deficiency anemia group (IDA).
(1) The expression of TfR1 mRNA in N group was 0.4813 +/- 0.1891, in ID group was 0. 6647 +/- 0.2788, and in IDA group was 0.9767 +/- 0.2858. There was significant difference between IDA group and N group or ID group (t = 0.002, P < 0.01 or t = 0.028, P < 0.05), and was no difference between ID group and N group (t = 0.117, P > 0.05). (2) The expression of IRP1 mRNA in N group was 0.2616 +/- 0.0785, in ID group was 0.3696 +/- 0.1801, and in IDA group was 0.3971 +/- 0.0902 and was no difference among the three groups (F = 1.845, P = 0.179).
The expression of TfR1 mRNA is increased when maternal iron deficiency progressed while there is no change in the expression of IRP1 mRNA in the placentae of TfR1 mRNA indicated that IRP1 takes part in the regulation of placenta iron transport.
研究不同母体铁状态下足月胎盘中转铁蛋白受体1（TfR1）和铁调节蛋白1（IRP1）的mRNA表达，探讨胎盘铁转运及调控机制。
采用逆转录聚合酶链反应（RT-PCR）检测正常组（N）、缺铁组（ID）和缺铁性贫血组（IDA）足月胎盘组织中TfR1和IRP1的mRNA水平。
（1）N组TfR1 mRNA表达量为0.4813±0.1891，ID组为0.6647±0.2788，IDA组为0.9767±0.2858。IDA组与N组或ID组比较差异有统计学意义（t=0.002，P<0.01或t=0.028，P<0.05），ID组与N组比较差异无统计学意义（t=0.117，P>0.05）。（2）N组IRP1 mRNA表达量为0.2616±0.0785，ID组为0.3696±0.1801，IDA组为0.3971±0.0902，三组比较差异无统计学意义（F=1.845，P=0.179）。
母体缺铁进展时TfR1 mRNA表达增加，而IRP1 mRNA表达无变化，提示IRP1参与胎盘铁转运的调节。
