Chanawong Aroonwadee, Lulitanond Aroonlug, Kaewkes Wanlop, Lulitanond Viraphong, Srigulbutr Sukanya, Homchampa Preecha
Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Southeast Asian J Trop Med Public Health. 2007 May;38(3):493-500.
This study presents updates on molecular epidemiology of extended-spectrum beta-lactamases (ESBLs) in clinical isolates of Enterobacteriaceae from Srinagarind Hospital, Khon Kaen University, Thailand. All isolates were screened for the presence of ESBL genes, bla(TEM), bla(SHV), bla(VEB) and bla(CTX-M), using PCR followed by nucleotide sequence determination. The results revealed that beta-lactamase genes among 48 isolates collected between 1998 and 1999 were bla(SHV) (79%), bla(CTX-M-9) (52%), bla(TEM-1) (48%) and bla(VEB) (33%), whereas those found in 52 isolates collected in 2003 were bla(TEM-1) (79%), bla(CTX-M-15) (44%), bla(SHV) (36%), bla(VEB) (36%), bla(CTX -M-14) (11%) and bla(CTX-M-9) (10%). In addition, 45 isolates carried at least two different ESBL genes. Using PCR, part of insertion sequence ISEcpl was found in the upstream regions of bla(CTX-M-14) and bla(CTX-M-15). ERIC-PCR analysis revealed that most ESBL-producing isolates were of different strains. This is the first report of CTX-M-9, CTX-M-14 and CTX-M-15 beta-lactamase genes in Enterobacteriaceae in Thailand.
本研究展示了泰国孔敬大学诗里那琳医院临床分离的肠杆菌科细菌中广谱β-内酰胺酶（ESBLs）分子流行病学的最新情况。使用聚合酶链反应（PCR）并随后进行核苷酸序列测定，对所有分离株进行ESBL基因bla(TEM)、bla(SHV)、bla(VEB)和bla(CTX-M)的筛查。结果显示，1998年至1999年收集的48株分离株中的β-内酰胺酶基因分别为bla(SHV)（79%）、bla(CTX-M-9)（52%）、bla(TEM-1)（48%）和bla(VEB)（33%），而2003年收集的52株分离株中的β-内酰胺酶基因分别为bla(TEM-1)（79%）、bla(CTX-M-15)（44%）、bla(SHV)（36%）、bla(VEB)（36%）、bla(CTX -M-14)（11%）和bla(CTX-M-9)（10%）。此外，45株分离株携带至少两种不同的ESBL基因。通过PCR，在bla(CTX-M-14)和bla(CTX-M-15)的上游区域发现了部分插入序列ISEcpl。肠杆菌重复间区序列聚合酶链反应（ERIC-PCR）分析表明，大多数产ESBL的分离株属于不同菌株。这是泰国肠杆菌科细菌中CTX-M-9、CTX-M-14和CTX-M-15β-内酰胺酶基因的首次报道。
