Müller S
Department of Environmental Microbiology, UFZ, Helmholtz Centre for Environmental Research, Leipzig-Halle, Leipzig, Germany.
Cell Prolif. 2007 Oct;40(5):621-39. doi: 10.1111/j.1365-2184.2007.00465.x.
Analyses of DNA pattern provide an excellent tool to determine activity states of bacteria. Bacterial cell cycle behaviour is generally different from the eukaryotic one and is pre-determined by the bacteria's diversity within the phylogenetic tree, and their metabolic traits. As a result, every species creates its specific proliferation pattern that differs from every other one. Up to now, just few bacterial species have been investigated and little information is available concerning DNA cycling even in already known species. This prevents understanding of the complexity and diversity of ongoing bacterial interactions in many ecosystems or in biotechnology. Flow cytometry is the only possible technique to shed light on the dynamics of bacterial communities and DNA patterns will help to unlock the hidden principles of their life. This review provides basic knowledge about the molecular background of bacterial cell cycling, discusses modes of cell cycle phases and presents techniques to both obtain DNA patterns and to combine the contained information with physiological cell states.
DNA模式分析为确定细菌的活性状态提供了一个出色的工具。细菌的细胞周期行为通常与真核生物不同，并且由细菌在系统发育树中的多样性及其代谢特征预先决定。因此，每个物种都形成了其特定的增殖模式，与其他物种不同。到目前为止，仅对少数细菌物种进行了研究，即使对于已知物种，有关DNA循环的信息也很少。这妨碍了对许多生态系统或生物技术中正在进行的细菌相互作用的复杂性和多样性的理解。流式细胞术是揭示细菌群落动态的唯一可行技术，而DNA模式将有助于揭示其生命的隐藏原理。本综述提供了有关细菌细胞循环分子背景的基础知识，讨论了细胞周期阶段的模式，并介绍了获取DNA模式以及将所含信息与生理细胞状态相结合的技术。
