Cooper Stephen
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI 48109-0620, USA.
Mol Microbiol. 2006 Oct;62(2):303-7. doi: 10.1111/j.1365-2958.2006.05342.x.
Bates and Kleckner have recently proposed that bacterial cell division is a licensing agent for a subsequent initiation of DNA replication. They also propose that initiation mass for DNA replication is not constant. These two proposals do not take into account older data showing that initiation of DNA replication can occur prior to the division event. This critical analysis is derived from measurements of DNA replication during the division cycle in cells growing at different, and more rapid, growth rates. Furthermore, mutants impaired in division can initiate DNA synthesis. The data presented by Bates and Kleckner do not support the proposal that initiation mass is variable, and the proposed pattern of DNA replication during the division cycle of the K12 cells analysed is not consistent with prior data on the pattern of DNA replication during the division cycle.
贝茨和克莱克纳最近提出，细菌细胞分裂是随后启动DNA复制的许可因子。他们还提出，DNA复制的起始量并非恒定不变。这两个提议没有考虑到更早的数据，这些数据表明DNA复制的起始可以在分裂事件之前发生。这一批判性分析源自对处于不同且更快生长速率下生长的细胞在分裂周期中DNA复制的测量。此外，在分裂中受损的突变体能够启动DNA合成。贝茨和克莱克纳所呈现的数据并不支持起始量可变这一提议，并且所分析的K12细胞在分裂周期中的DNA复制模式提议与之前关于DNA复制在分裂周期中的模式的数据不一致。
