van Schie Chris C N, Ament Kai, Schmidt Axel, Lange Theo, Haring Michel A, Schuurink Robert C
Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1098 SM Amsterdam, The Netherlands.
Plant J. 2007 Nov;52(4):752-62. doi: 10.1111/j.1365-313X.2007.03273.x. Epub 2007 Sep 18.
Geranyl diphosphate synthase (GPS) is generally considered to be responsible for the biosynthesis of monoterpene precursors only. However, reduction of LeGPS expression in tomato (Lycopersicon esculentum) by virus-induced gene silencing resulted in severely dwarfed plants. Further analysis of these dwarfed plants revealed a decreased gibberellin content, whereas carotenoid and chlorophyll levels were unaltered. Accordingly, the phenotype could be rescued by application of gibberellic acid. The dwarfed phenotype was also obtained in Arabidopsis thaliana plants transformed with RNAi constructs of AtGPS. These results link geranyl diphosphate (GPP) to the gibberellin biosynthesis pathway. They also demand a re-evaluation of the role of GPS in precursor synthesis for other di-, tri-, tetra- and/or polyterpenes and their derivatives.
香叶基二磷酸合酶（GPS）通常被认为仅负责单萜前体的生物合成。然而，通过病毒诱导的基因沉默降低番茄（Lycopersicon esculentum）中LeGPS的表达会导致植株严重矮化。对这些矮化植株的进一步分析显示赤霉素含量降低，而类胡萝卜素和叶绿素水平未改变。因此，施用赤霉酸可以挽救该表型。在用AtGPS的RNAi构建体转化的拟南芥植株中也获得了矮化表型。这些结果将香叶基二磷酸（GPP）与赤霉素生物合成途径联系起来。它们还要求重新评估GPS在其他二萜、三萜、四萜和/或多萜及其衍生物的前体合成中的作用。
