Elberling J, Skov P S, Mosbech H, Holst H, Dirksen A, Johansen J D
The Danish Research Centre for Chemical Sensitivities, Gentofte University Hospital, Gentofte, Denmark.
Clin Exp Allergy. 2007 Nov;37(11):1676-80. doi: 10.1111/j.1365-2222.2007.02824.x. Epub 2007 Sep 17.
Environmental perfume exposure may cause respiratory symptoms. Individuals with asthma and perfume contact allergy report such symptoms more frequently than others. However, immunologic mechanisms have not been demonstrated and the symptoms are not associated with IgE-mediated allergy. The study aimed to investigate whether basophils from patients with respiratory symptoms related to perfume released more histamine in the presence of perfume as compared with healthy volunteers.
Histamine release was measured by the glass fibre method. Blood was obtained from healthy volunteers (n=20) and patients with respiratory symptoms related to perfume (n=17) attending a dermatological outpatient clinic for patch testing. The effect of an international brand perfume was investigated using the basophil histamine release test with perfume. Furthermore, basophils from a healthy non-atopic donor were incubated with participant's sera and histamine release induced by perfume was measured.
In both groups incremental perfume concentrations showed a positive and significant (P<0.001) dose-response effect on the release of histamine. At the highest perfume concentration, the basophils released significantly (P<0.05) more histamine in patients as compared with healthy volunteers. No difference was found between the groups when sera were incubated with basophils from a healthy non-atopic donor.
Perfume induces a dose-dependent non-IgE-mediated release of histamine from human peripheral blood basophils. Increased basophil reactivity to perfume was found in patients with respiratory symptoms related to perfume.
接触环境中的香水可能会引发呼吸道症状。哮喘患者和对香水接触过敏的个体比其他人更频繁地报告此类症状。然而，免疫机制尚未得到证实，且这些症状与IgE介导的过敏无关。本研究旨在调查与香水相关的呼吸道症状患者的嗜碱性粒细胞在接触香水时是否比健康志愿者释放更多组胺。
采用玻璃纤维法测量组胺释放。从健康志愿者（n = 20）和因斑贴试验前往皮肤科门诊就诊的与香水相关的呼吸道症状患者（n = 17）中采集血液。使用嗜碱性粒细胞组胺释放试验研究一种国际品牌香水的作用。此外，将来自健康非特应性供体的嗜碱性粒细胞与参与者的血清一起孵育，并测量香水诱导的组胺释放。
在两组中，香水浓度的增加对组胺释放均显示出正向且显著（P < 0.001）的剂量反应效应。在最高香水浓度下，与健康志愿者相比，患者的嗜碱性粒细胞释放的组胺显著更多（P < 0.05）。当血清与来自健康非特应性供体的嗜碱性粒细胞一起孵育时，两组之间未发现差异。
香水可诱导人外周血嗜碱性粒细胞以剂量依赖的方式非IgE介导释放组胺。在与香水相关的呼吸道症状患者中发现嗜碱性粒细胞对香水的反应性增加。
