Lin Zheng-Mei, Qin Wei, Zhang Nian-Hua, Xiao Lin, Ling Jun-Qi
Department of Operative and Endodontics, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, China.
J Endod. 2007 Aug;33(8):930-5. doi: 10.1016/j.joen.2007.03.010. Epub 2007 May 9.
Recombinant human bone morphogenetic protein-7 (BMP-7) has been shown to stimulate new reparative dentin formation in animal models. However, little is known about whether BMP-7 could promote the odontoblast-like differentiation and the formation of mineralized nodules in human dental pulp cells. Here, we reported that the infection with adenovirus-BMP-7 (Ad-BMP-7), a BMP-7-expressing adenoviral vector, induced the expression of BMP-7 in primarily cultured human dental pulp cells in the long term with little effect on their proliferation and viability. Importantly, BMP-7 expression significantly increased alkaline phosphatase activity and induced the dentin sialophosphoprotein expression in a dose- and time-dependent manner, suggesting that BMP-7 promoted the odontoblast differentiation. Furthermore, BMP-7 expression stimulated the formation of many mineralized dentin-like calcified nodules. Our data suggest that Ad-BMP-7-mediated BMP-7 expression can promote the differentiation of human pulp cells into odontoblast-like cells and mineralization in vitro, which may provide insight for the design of new gene therapy for the pulp capping in the clinic.
重组人骨形态发生蛋白-7（BMP-7）已被证明能在动物模型中刺激新的修复性牙本质形成。然而，关于BMP-7是否能促进人牙髓细胞向成牙本质细胞样分化以及矿化结节的形成，人们了解甚少。在此，我们报告，用表达BMP-7的腺病毒载体腺病毒-BMP-7（Ad-BMP-7）感染，能长期诱导原代培养的人牙髓细胞中BMP-7的表达，且对其增殖和活力影响很小。重要的是，BMP-7表达显著增加碱性磷酸酶活性，并以剂量和时间依赖性方式诱导牙本质涎磷蛋白表达，表明BMP-7促进了成牙本质细胞分化。此外，BMP-7表达刺激了许多矿化牙本质样钙化结节的形成。我们的数据表明，Ad-BMP-7介导的BMP-7表达能促进人牙髓细胞在体外分化为成牙本质细胞样细胞并矿化，这可能为临床上牙髓盖髓新基因治疗的设计提供思路。
