Nozoe Tadahiro, Oyama Tsunehiro, Takenoyama Mistuhiro, Hanagiri Takeshi, Sugio Kenji, Yasumoto Kosei
Second Department of Surgery, University of Occupational and Environmental Health, Kitakyushu, Japan.
Oncology. 2006;71(5-6):402-10. doi: 10.1159/000108611. Epub 2007 Sep 18.
p27kip1 belongs to the KIP/CIP family of cyclin-dependent kinase inhibitors and is considered to be a tumor suppressor. Involucrin has been known as a marker of differentiation of squamous cell carcinoma (SCC). The aim of this study was to evaluate the clinicopathologic significance of the expression of p27 and involucrin in esophageal SCC.
Immunohistochemical expression of p27 and involucrin was examined in 70 specimens of esophageal SCC. The correlation of the expression of these proteins and clinicopathologic features was evaluated.
Cellular differentiation in esophageal SCC was significantly correlated with the expression of p27 and involucrin (p = 0.010 and p = 0.002, respectively). Among well, moderately and poorly differentiated SCCs, 45.8 +/- 21.6, 20.0 +/- 15.0 and 10.6 +/- 9.1% of carcinoma cells expressed involucrin, respectively (p < 0.0001 for well vs. poorly, p < 0.0001 for well vs. moderately, and p = 0.042 for moderately vs. poorly). There existed a more powerful statistical difference regarding the histological grade between SCCs with the expression of both p27 and involucrin and tumors with other expression patterns (p = 0.0001).
Expression of both p27 and involucrin can be a powerful biological marker of cellular differentiation of esophageal SCC.
p27kip1属于细胞周期蛋白依赖性激酶抑制剂的KIP/CIP家族，被认为是一种肿瘤抑制因子。外皮蛋白一直被视为鳞状细胞癌（SCC）分化的标志物。本研究的目的是评估p27和外皮蛋白在食管SCC中表达的临床病理意义。
检测70例食管SCC标本中p27和外皮蛋白的免疫组化表达情况。评估这些蛋白表达与临床病理特征之间的相关性。
食管SCC中的细胞分化与p27和外皮蛋白的表达显著相关（分别为p = 0.010和p = 0.002）。在高分化、中分化和低分化SCC中，分别有45.8±21.6%、20.0±15.0%和10.6±9.1%的癌细胞表达外皮蛋白（高分化与低分化相比p < 0.0001，高分化与中分化相比p < 0.0001，中分化与低分化相比p = 0.042）。在同时表达p27和外皮蛋白的SCC与具有其他表达模式的肿瘤之间，关于组织学分级存在更强有力的统计学差异（p = 0.0001）。
p27和外皮蛋白的表达均可成为食管SCC细胞分化的有力生物学标志物。
