Vala Helena, Carvalho Tânia, Pinto Carlos, Pereira Maria A, Mesquita João R, C Peleteiro Maria, Ferrer Lluís, Fondevila Dolores
Agrarian School of the Polytechnic Institute of Viseu, Quinta da Alagoa-Estrada de Nelas Ranhados, 3500-606 Viseu, Portugal.
Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal.
Vet Sci. 2020 May 29;7(2):70. doi: 10.3390/vetsci7020070.
Bovine Ocular Squamous Cell Carcinoma is considered the most common bovine tumour, causing significant economic losses, mainly by abattoir condemnations. To obtain a better insight into the genesis and neoplastic transformation, 19 samples collected at slaughter from Holstein Friesian cattle and diagnosed as Ocular Squamous Cell Carcinoma were studied. Tumours were histologically classified into three categories: poorly (26.3%), moderately (26.3%), and well differentiated (47.4%). Expression of keratins (MNF116 and LP34) and of cornified envelope precursors (involucrin and profilaggrin) was studied. Expression of MNF116 was observed in all carcinomas. LP34 immunostaining was seen in all but three carcinomas, one from each degree. Involucrin immunoreaction was observed in all but one poorly differentiated carcinoma. Profilaggrin was present in only two moderately differentiated carcinomas, in all but one well differentiated, and in all but one poorly differentiated. MNF116 is a useful marker to confirm the epithelial origin of the tumour and stain most neoplastic cells in these tumours. The expression of involucrin and LP34 demonstrates that, in all tumours, cells have reached the final program of differentiation, regardless of the grade. The expression of profilaggrin could indicate molecular changes during malignant transformation but their expression does not seem to be of diagnostic value.
牛眼鳞状细胞癌被认为是最常见的牛肿瘤，主要通过屠宰场拒收造成重大经济损失。为了更好地了解其发生和肿瘤转化过程，对19份从荷斯坦弗里生牛屠宰时采集并诊断为眼鳞状细胞癌的样本进行了研究。肿瘤在组织学上分为三类：低分化（26.3%）、中分化（26.3%）和高分化（47.4%）。研究了角蛋白（MNF116和LP34）以及角质包膜前体（内披蛋白和聚丝蛋白）的表达情况。在所有癌组织中均观察到MNF116的表达。除了三个癌组织（每个分化程度各一个）外，在其他所有癌组织中均可见LP34免疫染色。除了一个低分化癌组织外，在其他所有癌组织中均观察到内披蛋白免疫反应。聚丝蛋白仅在两个中分化癌组织、除一个外的所有高分化癌组织以及除一个外的所有低分化癌组织中存在。MNF116是确认肿瘤上皮起源并对这些肿瘤中的大多数肿瘤细胞进行染色有用的标志物。内披蛋白和LP34的表达表明，在所有肿瘤中，无论分级如何，细胞都已达到最终分化程序。聚丝蛋白的表达可能表明恶性转化过程中的分子变化，但其表达似乎没有诊断价值。
