Melgarejo E, Medina M A, Sánchez-Jiménez F, Botana L M, Domínguez M, Escribano L, Orfao A, Urdiales J L
Procel Lab, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, and CIBERER, 29071, Málaga, Spain.
Cell Mol Life Sci. 2007 Oct;64(19-20):2690-701. doi: 10.1007/s00018-007-7331-4.
Mast cells are multipotent effector cells of the immune system. They are able to induce and enhance angiogenesis via multiple pathways. (-)-Epigallocatechin-3-gallate (EGCG), a major component of green tea and a putative chemopreventive agent, was reported to inhibit tumor invasion and angiogenesis, processes that are essential for tumor growth and metastasis. Using the human mast cell line HMC-1 and commercial cDNA macroarrays, we evaluated the effect of EGCG on the expression of angiogenesis-related genes. Our data show that among other effects, EGCG treatment reduces expression of two integrins (alpha5 and beta3) and a chemokine (MCP1), resulting in a lower adhesion of mast cells associated with a decreased potential to produce signals eliciting monocyte recruitment. These effects on gene expression levels are functionally validated by showing inhibitory effects in adhesion, aggregation, migration and recruitment assays.
肥大细胞是免疫系统的多能效应细胞。它们能够通过多种途径诱导和增强血管生成。（-）-表没食子儿茶素-3-没食子酸酯（EGCG）是绿茶的主要成分，也是一种公认的化学预防剂，据报道它能抑制肿瘤侵袭和血管生成，而这两个过程对肿瘤生长和转移至关重要。我们使用人肥大细胞系HMC-1和商业cDNA微阵列，评估了EGCG对血管生成相关基因表达的影响。我们的数据表明，除其他作用外，EGCG处理可降低两种整合素（α5和β3）和一种趋化因子（MCP1）的表达，导致肥大细胞的黏附性降低，同时产生引发单核细胞募集信号的潜力也降低。通过在黏附、聚集、迁移和募集试验中显示出抑制作用，对这些基因表达水平的影响在功能上得到了验证。
