Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, and CIBER de Enfermedades Raras (CIBERER), Málaga, Spain.
Br J Pharmacol. 2009 Dec;158(7):1705-12. doi: 10.1111/j.1476-5381.2009.00452.x.
Monocytes/macrophages are an important population of immune inflammatory cells that have diverse effector functions in which their mobility and adhesion play a very relevant role. Epigallocatechin gallate (EGCG), a major component of green tea, has been reported to have anti-allergic and anti-inflammatory activities, but its effects on monocytes remain to be determined. Here we investigated the effects of EGCG on the migration and adhesion of monocytes.
We used a human monocyte cell line (THP-1) to analyse the effects of treatment with EGCG under non-cytotoxic conditions on the expression levels of the monocyte chemotactic protein-1 (MCP-1) and of the MCP-1 receptor (CCR2) and on the activation of beta1 integrin. A functional validation was carried out by evaluating the inhibitory effect of EGCG on monocyte adhesiveness and migration in vitro.
Treatment of THP-1 cells with EGCG decreased MCP-1 and CCR2 gene expression, together with MCP-1 secretion and CCR2 expression at the cell surface. EGCG also inhibited beta1 integrin activation. The effects on these molecular targets were in agreement with the EGCG-induced inhibition of THP-1 migration in response to MCP-1 and adhesion to fibronectin.
Under our experimental conditions, EGCG treatment inhibited the migration and adhesion of monocytes. These inhibitory effects of EGCG on monocyte function should be considered as a promising new anti-inflammatory response with a potential therapeutic role in the treatment of inflammation-dependent diseases.
单核细胞/巨噬细胞是一种重要的免疫炎性细胞群体，具有多种效应功能，其中它们的迁移和黏附作用非常重要。表没食子儿茶素没食子酸酯（EGCG）是绿茶的主要成分之一，已被报道具有抗过敏和抗炎活性，但它对单核细胞的影响仍有待确定。在这里，我们研究了 EGCG 对单核细胞迁移和黏附的影响。
我们使用人单核细胞系（THP-1）来分析在非细胞毒性条件下，EGCG 处理对单核细胞趋化蛋白-1（MCP-1）和 MCP-1 受体（CCR2）表达水平的影响，以及对β1 整合素的激活作用。通过评估 EGCG 对体外单核细胞黏附和迁移的抑制作用，进行了功能验证。
EGCG 处理 THP-1 细胞可降低 MCP-1 和 CCR2 基因表达，同时降低 MCP-1 分泌和细胞表面 CCR2 表达。EGCG 还抑制β1 整合素的激活。这些分子靶点的作用与 EGCG 诱导的 THP-1 迁移抑制作用一致，该作用对 MCP-1 有反应，对纤维连接蛋白的黏附也有抑制作用。
在我们的实验条件下，EGCG 处理抑制了单核细胞的迁移和黏附。EGCG 对单核细胞功能的这些抑制作用应被视为一种有前途的新抗炎反应，在炎症相关疾病的治疗中具有潜在的治疗作用。
