Márquez-Velasco R, Massó F, Hernández-Pando R, Montaño L F, Springall R, Amezcua-Guerra L M, Bojalil R
Ciencias Biológicas, UAM-Iztapalapa, Mexico City, Mexico.
Inflamm Res. 2007 Sep;56(9):385-90. doi: 10.1007/s00011-007-6116-4.
To explore the efficacy of prophylactic oral lipopolysaccharide (LPS) in sepsis induced by cecal ligation and puncture (CLP).
Male Balb/c mice. LPS serotype O55: B5
Mice were treated every 4 days for a total of 5 times with 50 mug of LPS by intraperitoneal (IP) or oral (O) routes. Treatment was stopped one week prior to CLP. Control (C) groups received the vehicle orally, and sham (S) groups were used as reference.
Histopathology was performed to determine inflammation in liver and lung. Serum cytokines were measured by ELISA, and TNFalpha tissue expression by RTPCR. Antibodies against LPS were measured by ELISA.
Administration of LPS by the oral route significantly increased survival (p<0.05) of mice in association with a reduction of Kupffer cells in liver, pulmonary edema in lung, shorter or delayed TNFalpha expression in target organs, a trend to decreased IFN gamma and increased IL-10 serum levels, and a notable increase in the production of specific IgM anti-LPS antibodies.
LPS by oral route protected against CLP. The underlying mechanisms could be the modulation of the proinflammatory response and an increased production of IgM anti-LPS antibodies.
探讨预防性口服脂多糖（LPS）对盲肠结扎穿孔（CLP）诱导的脓毒症的疗效。
雄性Balb/c小鼠。LPS血清型O55:B5
小鼠通过腹腔内（IP）或口服（O）途径，每4天用50微克LPS处理，共5次。在CLP前一周停止处理。对照组（C）经口给予赋形剂，假手术组（S）作为对照。
进行组织病理学检查以确定肝脏和肺中的炎症。通过酶联免疫吸附测定（ELISA）测量血清细胞因子，通过逆转录聚合酶链反应（RTPCR）测量肿瘤坏死因子α（TNFα）的组织表达。通过ELISA测量抗LPS抗体。
口服LPS可显著提高小鼠的存活率（p<0.05），同时肝脏中库普弗细胞减少、肺部肺水肿减轻、靶器官中TNFα表达缩短或延迟、干扰素γ（IFNγ）血清水平有降低趋势以及白细胞介素10（IL-10）血清水平升高，并且抗LPS特异性IgM抗体的产生显著增加。
口服LPS可预防CLP。潜在机制可能是对促炎反应的调节以及抗LPS IgM抗体产生增加。
